Parameters Governing the Community Structure and Element Turnover in Kermadec Volcanic Ash and Hydrothermal Fluids as Monitored by Inorganic Electron Donor Consumption, Autotrophic CO2 Fixation and 16S Tags of the Transcriptome in Incubation Experiments
The microbial community composition and its functionality was assessed for hydrothermal fluids and volcanic ash sediments from Haungaroa and hydrothermal fluids from the Brothers volcano in the Kermadec island arc (New Zealand). The Haungaroa volcanic ash sediments were dominated by epsilonproteobac...
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Published in | Frontiers in microbiology Vol. 10; p. 2296 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
09.10.2019
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Subjects | |
Online Access | Get full text |
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Summary: | The microbial community composition and its functionality was assessed for hydrothermal fluids and volcanic ash sediments from Haungaroa and hydrothermal fluids from the Brothers volcano in the Kermadec island arc (New Zealand). The Haungaroa volcanic ash sediments were dominated by epsilonproteobacterial
Sulfurovum
sp. Ratios of electron donor consumption to CO
2
fixation from respective sediment incubations indicated that sulfide oxidation appeared to fuel autotrophic CO
2
fixation, coinciding with thermodynamic estimates predicting sulfide oxidation as the major energy source in the environment. Transcript analyses with the sulfide-supplemented sediment slurries demonstrated that
Sulfurovum
prevailed in the experiments as well. Hence, our sediment incubations appeared to simulate environmental conditions well suggesting that sulfide oxidation catalyzed by
Sulfurovum
members drive biomass synthesis in the volcanic ash sediments. For the Haungaroa fluids no inorganic electron donor and responsible microorganisms could be identified that clearly stimulated autotrophic CO
2
fixation. In the Brothers hydrothermal fluids
Sulfurimonas
(49%) and
Hydrogenovibrio/Thiomicrospira
(15%) species prevailed. Respective fluid incubations exhibited highest autotrophic CO
2
fixation if supplemented with iron(II) or hydrogen. Likewise catabolic energy calculations predicted primarily iron(II) but also hydrogen oxidation as major energy sources in the natural fluids. According to transcript analyses with material from the incubation experiments
Thiomicrospira/Hydrogenovibrio
species dominated, outcompeting
Sulfurimonas
. Given that experimental conditions likely only simulated environmental conditions that cause
Thiomicrospira/Hydrogenovibrio
but not
Sulfurimonas
to thrive, it remains unclear which environmental parameters determine
Sulfurimonas’
dominance in the Brothers natural hydrothermal fluids. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by: Mark Alexander Lever, ETH Zürich, Switzerland Present address: Stefanie Böhnke and Mirjam Perner, Geomicrobiology, GEOMAR, Helmholtz Centre for Ocean Research Kiel, Kiel, Germany Sylvia G. Sander, Marine Environment Studies Laboratory, International Atomic Energy Agency, Monaco, Monaco This article was submitted to Extreme Microbiology, a section of the journal Frontiers in Microbiology Reviewed by: Kathleen Scott, University of South Florida, Tampa, United States; Ida Helene Steen, University of Bergen, Norway |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2019.02296 |