Fluorescent Single-Core and Multi-Core Nanoprobes as Cell Trackers and Magnetic Nanoheaters

• Published in: Magnetochemistry Vol. 8; no. 8; p. 83
• Main Authors: García Acevedo, Pelayo, González Gómez, Manuel A., Arnosa Prieto, Ángela, De Castro Alves, Lisandra, Seco Gudiña, Román, Piñeiro, Yolanda, Rivas, José
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.08.2022
• Subjects: Analysis; Biomedical materials; cell tracking; Chemical synthesis; Chitosan; Contrast agents; Cost analysis; Efficiency; Fever; Fluorescein; Fluorescence; Hyperthermia; Identification and classification; Inorganic coatings; iron oxide magnetic nanoparticles; Iron oxides; Magnetic fields; magnetic hyperthermia; Magnetic materials; Magnetic properties; Magnetic resonance imaging; Medical imaging; Methods; nanomagnetism; Nanomaterials; Nanoparticles; nanowarming; Optical properties; Protective coatings; Quantum dots; Rhodamine; Silicon dioxide; Spain
• ISSN: 2312-7481; 2312-7481
• DOI: 10.3390/magnetochemistry8080083

Iron oxide magnetic nanoparticles (MNPs) have been widely studied due to their versatility for diagnosis, tracking (magnetic resonance imaging (MRI)) and therapeutic (magnetic hyperthermia and drug delivery) applications. In this work, iron oxide MNPs with different single-core (8–40 nm) and multi-core (140–200 nm) structures were synthesized and functionalized by organic and inorganic coating materials, highlighting their ability as magnetic nanotools to boost cell biotechnological procedures. Single core Fe3O4@PDA, Fe3O4@SiO2-FITC-SiO2 and Fe3O4@SiO2-RITC-SiO2 MNPs were functionalized with fluorescent components with emission at different wavelengths, 424 nm (polydopamine), 515 (fluorescein) and 583 nm (rhodamine), and their ability as transfection and imaging agents was explored with HeLa cells. Moreover, different multi-core iron oxide MNPs (Fe3O4@CS, Fe3O4@SiO2 and Fe3O4@Citrate) coated with organic (citrate and chitosan, CS) and inorganic (silica, SiO2) shells were tested as efficient nanoheaters for magnetic hyperthermia applications for mild thermal heating procedures as an alternative to simple structures based on single-core MNPs. This work highlights the multiple abilities offered by the synergy of the use of external magnetic fields applied on MNPs and their application in different biomedical approaches.