Exploring the Relationship Between Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17
Characterizing and engineering microbial communities for lignocellulosic biofuel production has received widespread attention. Previous research has established that Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17 coculture significantly improves overall cellulosic...
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Published in | Frontiers in microbiology Vol. 10; p. 2035 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
10.09.2019
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Subjects | |
Online Access | Get full text |
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Summary: | Characterizing and engineering microbial communities for lignocellulosic biofuel production has received widespread attention. Previous research has established that
Clostridium thermocellum
JN4 and
Thermoanaerobacterium thermosaccharolyticum
GD17 coculture significantly improves overall cellulosic biofuel production efficiency. Here, we investigated this interaction and revealed the mechanism underlying the improved efficiency observed. In contrast to the previously reported mutualistic relationship, a harmful effect toward
C
.
thermocellum
JN4 was observed in these microbial consortia. Although
T. thermosaccharolyticum
GD17 relieves the carbon catabolite repression of
C
.
thermocellum
JN4 regarding obtaining more cellobiose or glucose released from lignocellulose,
T. thermosaccharolyticum
GD17 significantly hampers the growth of
C
.
thermocellum
JN4 in coculture. The increased formation of end products is due to the strong competitive metabolic advantage of
T. thermosaccharolyticum
GD17 over
C
.
thermocellum
JN4 in the conversion of glucose or cellobiose into final products. The possibility of controlling and rebalancing these microbial consortia to modulate cellulose degradation was achieved by adding
T. thermosaccharolyticum
GD17 stimulants into the system. As cellulolytic bacteria are usually at a metabolic disadvantage, these discoveries may apply to a large proportion of cellulosic biofuel-producing microbial consortia. These findings provide a reference for engineering efficient and modular microbial consortia for modulating cellulosic conversion. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Fengxue Xin, Nanjing Tech University, China; Zhiqiang Wen, Nanjing University of Science and Technology, China; Jiufu Qin, Technical University of Denmark, Denmark Edited by: Xiao-Jun Ji, Nanjing Tech University, China These authors have contributed equally to this work This article was submitted to Microbiotechnology, Ecotoxicology and Bioremediation, a section of the journal Frontiers in Microbiology |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2019.02035 |