A proteomic study of spike development inhibition in bread wheat

• Published in: Proteomics (Weinheim) Vol. 13; no. 17; pp. 2622 - 2637
• Main Authors: Zheng, Yong-Sheng, Guo, Jun-Xian, Zhang, Jin-Peng, Gao, Ai-Nong, Yang, Xin-Ming, Li, Xiu-Quan, Liu, Wei-Hua, Li, Li-Hui
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.09.2013; Wiley Subscription Services, Inc
• Subjects: 2DE; Autonomous pathway; Bread wheat; Carrier Proteins - analysis; Carrier Proteins - biosynthesis; Cold reponses; Cold-Shock Response; Databases, Protein; Down-Regulation; Flowers - embryology; Flowers - growth & development; Gene Expression Regulation, Plant; Plant Proteins - analysis; Plant proteomics; Protein Folding; Proteomics - methods; Reactive Oxygen Species; Real-Time Polymerase Chain Reaction; Sequence Analysis, Protein; Spike-development inhibition; Tandem Mass Spectrometry; Triticum - embryology; Triticum - genetics; Triticum - growth & development; Triticum aestivum; Plant proteomics; Spike-development inhibition; 2DE; Autonomous pathway; Bread wheat; Cold reponses
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.201300026

Spike development in wheat is a complicated development process and determines the wheat propagation and survival. We report herein a proteomic study on the bread wheat mutant strain 5660M underlying spike development inhibition. A total of 121 differentially expressed proteins, which were involved in cold stress response, protein folding and assembly, cell‐cycle regulation, scavenging of ROS, and the autonomous pathway were identified using MS/MS and database searching. We found that cold responsive proteins were highly expressed in the mutant in contrast to those expressed in the wild‐type line. Particularly, the autonomous pathway protein FVE, which modulates flowering, was dramatically downregulated and closely related to the spike development inhibition phenotype of 5660M. A quantitative RT‐PCR study demonstrated that the transcription of the FVE and other six genes in the autonomous pathway and downstream flowering regulators were all markedly downregulated. The results indicate that spike development of 5660M cannot complete the floral transition. FVE might play an important role in the spikes development of the wheat. Our results provide the theory basis for studying floral development and transition in the reproductive growth period, and further analysis of wheat yield formation.