Isolation and characterization of microsatellite loci from the entomopathogenic hyphomycete, Paecilomyces fumosoroseus

Nine microsatellite markers were isolated from the entomopathogenic haploid fungus Paecilomyces fumosoroseus. Genetic diversity was assessed in 26 P. fumosoroseus isolates originated from the whitefly Bemisia tabaci collected in various geoclimatic areas. Eight loci were polymorphic with an observed...

Full description

Saved in:
Bibliographic Details
Published inMolecular ecology notes Vol. 5; no. 3; pp. 496 - 498
Main Authors Dalleau-Clouet, C, Gauthier, N, Risterucci, A.M, Bon, M.C, Fargues, J
Format Journal Article
LanguageEnglish
Published 01.09.2005
Subjects
alleles
alleles per locus
Bemisia tabaci
DNA primers
entomopathogenic fungi
genetic markers
genetic polymorphism
genetic variation
isolation
loci
microsatellite repeats
molecular sequence data
nucleotide sequences
Paecilomyces fumosoroseus
polymerase chain reaction
polymorphic loci
Online AccessGet more information

Cover

More Information
Summary:Nine microsatellite markers were isolated from the entomopathogenic haploid fungus Paecilomyces fumosoroseus. Genetic diversity was assessed in 26 P. fumosoroseus isolates originated from the whitefly Bemisia tabaci collected in various geoclimatic areas. Eight loci were polymorphic with an observed number of alleles ranging from two to six. The loci differentiated some isolates and group of isolates according to their geographical location, showing promise for the study of gene flow. All loci failed to give clear amplifications in P. fumosoroseus isolates from hosts other than B. tabaci. These microsatellite markers provide powerful tools for ecological, epidemiological, and population genetic studies.
Bibliography:http://dx.doi.org/10.1111/j.1471-8286.2005.00968.x
http://hdl.handle.net/10113/41102
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2005.00968.x