Chemiluminescent Probes Based on 1,2‐Dioxetane Structures For Bioimaging

• Published in: Chemistry, an Asian journal Vol. 17; no. 6; pp. e202200018 - n/a
• Main Authors: Wang, Yaling, Bian, Yongning, Chen, Xueqian, Su, Dongdong
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 14.03.2022
• Subjects: 1,2-dioxetane; biomarker; Biomarkers; Chemiluminescence; Chemiluminescence imaging; Chemiluminescent probes; Chemistry; Fluorescence; Heterocyclic Compounds, 1-Ring - chemistry; in vivo; Luminescence; Luminescent Measurements; Medical imaging; Noise sensitivity; 1,2-dioxetane; Chemiluminescence imaging; Chemiluminescent probes; biomarker; in vivo
• ISSN: 1861-4728; 1861-471X; 1861-471X
• DOI: 10.1002/asia.202200018

Chemiluminescent probes based on 1,2‐dioxetane scaffold are one of the most sensitive imaging modalities for detecting disease‐related biomarkers and can obtain more accurate biological information in cells and in vivo. Due to the elimination of external light excitation, the background autofluorescence problem in fluorescence technology can be effectively avoided, providing ultrahigh sensitivity and signal‐to‐noise ratio for various applications. In this review, we highlight a comprehensive but concise overview of activatable 1,2‐dioxetane‐based chemiluminescent probes by reporting significant advances in accurate detection and bioimaging. The design principles and applications for reactive species, enzymes, and other disease‐related biomarkers are systematically discussed and summarized. The challenges and potential prospects of chemiluminescent probes are also discussed to further promote the development of new chemiluminescence methods for biological analysis and diagnosis. 1,2‐Dioxetane‐based chemiluminescent probes activated by biomarkers have been widely studied in bioimaging due to their excellent properties. Recent progress and prospects in the design of activated 1,2‐dioxetane‐based chemiluminescent probes and their application in accurate detection and imaging of disease‐related biomarkers are reviewed.