Functional development and regeneration of hair cells in the zebrafish lateral line

• Published in: The Journal of physiology Vol. 599; no. 16; pp. 3913 - 3936
• Main Authors: Hardy, Katherine, Amariutei, Ana E., De Faveri, Francesca, Hendry, Aenea, Marcotti, Walter, Ceriani, Federico
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01.08.2021
• Subjects: afferent fibres; Animals; Danio rerio; development; Electrophysiology; Fertilization; Fibers; Fluorescent indicators; Hair cells; Hair Cells, Auditory; Hearing protection; imaging; Lateral line; Lateral Line System; Mechanoreceptors; neuromast; Neuromasts; otoxic compound; Regeneration; ribbon synapses; Sensory neurons; Vestibular system; Zebrafish; hair cells; development; ribbon synapses; otoxic compound; electrophysiology; regeneration; zebrafish; afferent fibres; imaging; neuromast
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1113/JP281522

Key points We investigated hair‐cell regeneration in the zebrafish lateral line following the application of the ototoxic compound copper. In early‐larval zebrafish (<10 days post‐fertilisation), regenerated hair cells drive action potentials (APs) in the afferent neurons 24 h post‐copper treatment (24 hpt). Full regeneration of the early‐larval neuromasts, the organs containing the hair cells, requires ∼48 h due to the progressive addition of hair cells and synaptic refinement. In older larval zebrafish, regenerated hair cells are active and drive afferent APs by 48 hpt, which is comparable to larvae, but the functional recovery of their neuromasts requires >120 hpt. Afferent terminals within the regenerating neuromast appear to initially contact supporting cells, and their complete ablation prevents the timely reappearance of supporting cells and hair cells. We conclude that the regeneration of zebrafish neuromasts is slower after the initial developmental stages, and that the afferent input plays a key role in driving this process. Hair cells are mechanosensory receptors responsible for transducing auditory and vestibular information into electrical signals, which are then transmitted with remarkable precision to afferent neurons. Different from mammals, the hair cells of lower vertebrates, including those present in the neuromasts of the zebrafish lateral line, regenerate following environmental or chemical insults. Here we investigate the time course of regeneration of hair cells in vivo using electrophysiology, two‐photon imaging and immunostaining applied to wild‐type and genetically encoded fluorescent indicator zebrafish lines. Functional hair cells drive spontaneous action potentials in the posterior lateral line afferent fibres, the frequency of which progressively increases over the first 10 days post‐fertilisation (dpf). Higher firing‐rate fibres are only observed from ∼6 dpf. Following copper treatment, newly formed hair cells become functional and are able to drive APs in the afferent fibres within 48 h in both early‐larval (≤8 dpf) and late‐larval (12–17 dpf) zebrafish. However, the complete functional regeneration of the entire neuromast is delayed in late‐larval compared to early‐larval zebrafish. We propose that while individual regenerating hair cells can rapidly become active, the acquisition of fully functional neuromasts progresses faster at early‐larval stages, a time when hair cells are still under development. At both ages, the afferent terminals in the regenerating neuromast appear to make initial contact with supporting cells. The ablation of the lateral line afferent neurons prevents the timely regeneration of supporting cells and hair cells. These findings indicate that the afferent system is likely to facilitate or promote the neuromast regeneration process. Key points We investigated hair‐cell regeneration in the zebrafish lateral line following the application of the ototoxic compound copper. In early‐larval zebrafish (<10 days post‐fertilisation), regenerated hair cells drive action potentials (APs) in the afferent neurons 24 h post‐copper treatment (24 hpt). Full regeneration of the early‐larval neuromasts, the organs containing the hair cells, requires ∼48 h due to the progressive addition of hair cells and synaptic refinement. In older larval zebrafish, regenerated hair cells are active and drive afferent APs by 48 hpt, which is comparable to larvae, but the functional recovery of their neuromasts requires >120 hpt. Afferent terminals within the regenerating neuromast appear to initially contact supporting cells, and their complete ablation prevents the timely reappearance of supporting cells and hair cells. We conclude that the regeneration of zebrafish neuromasts is slower after the initial developmental stages, and that the afferent input plays a key role in driving this process.