Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts

• Published in: Microbial cell factories Vol. 22; no. 1; p. 144
• Main Authors: Coradetti, Samuel T, Adamczyk, Paul A, Liu, Di, Gao, Yuqian, Otoupal, Peter B, Geiselman, Gina M, Webb-Robertson, Bobbie-Jo M, Burnet, Meagan C, Kim, Young-Mo, Burnum-Johnson, Kristin E, Magnuson, Jon, Gladden, John M
• Format: Journal Article
• Language: English
• Published: England BioMed Central 03.08.2023; Springer Science + Business Media; BMC
• Subjects: Alcohol; Alcohols; Assimilation; Batch culture; Biomass; Carbon; Carbon catabolite repression; Catabolism; Catabolite repression; Conversion; Fatty alcohol; Fungi; Gene regulation; Glucose; Growth rate; Metabolism; Mutation; Pentose; Phylogenetics; Plant biomass; Proteins; Proteomics; Rhodosporidium toruloides; Sugar; Transcription factors; Transcriptional regulation; Xylitol; Xylose; Xylose metabolism; Yeast; Yeasts; Carbon catabolite repression; Fatty alcohol; Transcriptional regulation; Xylose metabolism; Proteomics; Rhodosporidium toruloides
• ISSN: 1475-2859; 1475-2859
• DOI: 10.1186/s12934-023-02148-5

Efficient conversion of pentose sugars remains a significant barrier to the replacement of petroleum-derived chemicals with plant biomass-derived bioproducts. While the oleaginous yeast Rhodosporidium toruloides (also known as Rhodotorula toruloides) has a relatively robust native metabolism of pentose sugars compared to other wild yeasts, faster assimilation of those sugars will be required for industrial utilization of pentoses. To increase the rate of pentose assimilation in R. toruloides, we leveraged previously reported high-throughput fitness data to identify potential regulators of pentose catabolism. Two genes were selected for further investigation, a putative transcription factor (RTO4_12978, Pnt1) and a homolog of a glucose transceptor involved in carbon catabolite repression (RTO4_11990). Overexpression of Pnt1 increased the specific growth rate approximately twofold early in cultures on xylose and increased the maximum specific growth by 18% while decreasing accumulation of arabitol and xylitol in fast-growing cultures. Improved growth dynamics on xylose translated to a 120% increase in the overall rate of xylose conversion to fatty alcohols in batch culture. Proteomic analysis confirmed that Pnt1 is a major regulator of pentose catabolism in R. toruloides. Deletion of RTO4_11990 increased the growth rate on xylose, but did not relieve carbon catabolite repression in the presence of glucose. Carbon catabolite repression signaling networks remain poorly characterized in R. toruloides and likely comprise a different set of proteins than those mainly characterized in ascomycete fungi.