Effects of Aeration of Sawdust Cultivation Bags on Hyphal Growth of Lentinula edodes

• Published in: Mycobiology Vol. 40; no. 3; pp. 164 - 167
• Main Authors: Lee, H.Y., Chungbuk National University, Cheongju, Republic of Korea, Ham, E.J., Chungbuk National University, Cheongju, Republic of Korea, Yoo, Y.J., Jeollabuk-do Agricultural Research and Crop Management, Iksan, Republic of Korea, Kim, E.S., Pal-O Tech Ltd., Hwaseong, Republic of Korea, Shim, K.K., Pal-O Tech Ltd., Hwaseong, Republic of Korea, Kim, M.K., Jeonbuk National University, Iksan, Republic of Korea, Koo, C.D., Chungbuk National University, Cheongju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society of Mycology 01.09.2012; 한국균학회
• Subjects: concentration; Hyphal growth; LENTINULA EDODES; Medium weight loss; Sawdust bag cultivation; 생물학; CO2 concentration; Sawdust bag cultivation; Hyphal growth; Medium weight loss; Lentinula edodes
• ISSN: 1229-8093; 2092-9323
• DOI: 10.5941/myco.2012.40.3.164

The effects of aeration through lid filters on the hyphal growth of Lentinula edodes (oak mushroom) in sawdust cultivation bags were investigated. The aeration treatment levels were traditional 27 mm hole cotton plugs, cotton balls and combinations of seven hole sizes two hole positions (up and under) in the lids covering plastic bags containing 1.4 kg sawdust medium at 63% moisture that had been autoclaved for one hour and inoculated with sawdust spawn of L. edodes strain 921. Aeration treatment effects were measured based on the CO₂ concentration at the 15th wk, as well as the hyphal growth rate and degree of weight loss of bags every 14 days for 15 wk. In bags with traditional cotton plugs, the CO₂ concentration was 3.8±1.3%, daily mean hyphal growth was 2.3±0.6 mm and daily mean weight loss was 0.84±0.26 g. In the bags with 15 mm diameter holes, the CO₂ concentration was 6.0±1.6%, daily hyphal growth was 2.8±0.2 mm and daily weight loss was 0.86±0.4 g. The bags with 15 mm holes had a higher CO₂ concentration and lower water loss than bags with other hole sizes, but the hyphal growth was not significantly different from that of other bags. The weight loss of bags increased proportionally relative to the lid hole sizes. Taken together, these results indicate that traditional cotton plugs are economically efficient, but 15 mm hole lids are the most efficient at maintaining hyphal growth and controlling water loss while allowing CO₂ emissions.