Optimization of a hydrophobic solid-phase extraction interface for matrix-assisted laser desorption/ionization
Matrix‐assisted laser desorption/ionization (MALDI) probe surfaces derivatized with octadecanethiol (C18) can be used as hydrophobic solid‐phase extraction devices to isolate and desalt biopolymers directly on the probe surface. Using quantitative MALDI, it was possible to determine the approximate...
Saved in:
Published in | Journal of mass spectrometry. Vol. 33; no. 11; pp. 1141 - 1147 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
01.11.1998
Wiley |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Matrix‐assisted laser desorption/ionization
(MALDI) probe surfaces derivatized with octadecanethiol
(C18) can be used as hydrophobic
solid‐phase extraction devices to isolate and desalt
biopolymers directly on the probe surface. Using quantitative MALDI,
it was possible to determine the approximate amount of peptide that
bound to C18 surfaces and thus to calculate a surface
density. It was determined that the amount of peptide bound at the
probe surface was independent of the analyte concentration in the
immersion solution (from high‐ to sub‐ng
ml‐1 concentrations), but rather was dependent
on the immersion time of the surface as it was exposed to the analyte.
The capacity of C18‐derivatized probes to bind
biopolymers in fixed amounts frees the analyst from the necessity for
adjusting analyte concentration through multiple step procedures such
as serial dilution or vacuum drying. This time savings result in an
overall increase in the efficiency of the MALDI technique. © 1998
John Wiley & Sons, Ltd. |
---|---|
Bibliography: | National Institutes of Health - No. T32 AI07322 ark:/67375/WNG-VLT6GC6L-P National Science Foundation - No. 9626835 istex:69637A45808CE3A8B7E4156301A88CBAF81EC22E ArticleID:JMS732 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1076-5174 1096-9888 |
DOI: | 10.1002/(SICI)1096-9888(1998110)33:11<1141::AID-JMS732>3.0.CO;2-U |