Comparative proteomic analysis of early salt stress responsive proteins in roots and leaves of rice

• Published in: Proteomics (Weinheim) Vol. 14; no. 15; pp. 1759 - 1775
• Main Authors: Liu, Chih-Wei, Chang, Tao-Shan, Hsu, Yu-Kai, Wang, Arthur Z., Yen, Hung-Chen, Wu, Yung-Pei, Wang, Chang-Sheng, Lai, Chien-Chen
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.08.2014; Wiley Subscription Services, Inc
• Subjects: Abiotic stress; Chromatography, Liquid; Differentially expressed proteome; Electrophoresis, Gel, Two-Dimensional; Gel-based proteomics; LC-MS/MS; Oryza - chemistry; Oryza - metabolism; Oryza - physiology; Oryza sativa; Plant Leaves - chemistry; Plant Leaves - metabolism; Plant Proteins - analysis; Plant Proteins - chemistry; Plant proteomics; Plant Roots - chemistry; Plant Roots - metabolism; Proteins; Proteome - analysis; Proteome - chemistry; Proteome - drug effects; Proteomics - methods; Rice; Salinity; Salt; Salt stress; Salt-Tolerance - physiology; Sodium Chloride - pharmacology; Stress, Physiological - drug effects; Tandem Mass Spectrometry; Plant proteomics; LC-MS/MS; Gel-based proteomics; Salt stress; Differentially expressed proteome; Rice
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.201300276

Growth and productivity of rice (Oryza sativa L.) are severely affected by salinity. Understanding the mechanisms that protect rice and other important cereal crops from salt stress will help in the development of salt‐stress‐tolerant strains. In this study, rice seedlings of the same genetic species with various salt tolerances were studied. We first used 2DE to resolve the expressed proteome in rice roots and leaves and then used nanospray liquid chromatography/tandem mass spectrometry to identify the differentially expressed proteins in rice seedlings after salt treatment. The 2DE assays revealed that there were 104 differentially expressed protein spots in rice roots and 59 in leaves. Then, we identified 83 proteins in rice roots and 61 proteins in rice leaves by MS analysis. Functional classification analysis revealed that the differentially expressed proteins from roots could be classified into 18 functional categories while those from leaves could be classified into 11 functional categories. The proteins from rice seedlings that most significantly contributed to a protective effect against increased salinity were cysteine synthase, adenosine triphosphate synthase, quercetin 3‐O‐methyltransferase 1, and lipoxygenase 2. Further analysis demonstrated that the primary mechanisms underlying the ability of rice seedlings to tolerate salt stress were glycolysis, purine metabolism, and photosynthesis. Thus, we suggest that differentially expressed proteins may serve as marker group for the salt tolerance of rice.