Identification of a novel HLA-DRB1 allele, DRB10108, by sequence-based DRB typing in two siblings

• Published in: Tissue antigens Vol. 59; no. 4; pp. 350 - 351
• Main Authors: Martinez-Gallo, M., Martinez-Lostao, L., Martinez-Carretero, M.A., Gago, I., Maroto, P., Rodriguez-Sanchez, J L., De La Calle-Martin, O.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science, Ltd 01.04.2002
• Subjects: Alleles; Base Sequence; HLA-DR Antigens - genetics; HLA-DRB1 Chains; HLA-DRB101; Humans; sequence-based typing; Siblings
• ISSN: 0001-2815; 1399-0039
• DOI: 10.1034/j.1399-0039.2002.590421.x

: We report here a novel DRB1 allele identified during sequence‐based HLA‐DRB typing. This allele was detected during routine HLA typing of a patient and his family prior to bone marrow transplantation. The new allele, DRB1*0108, was found in the patient and in a brother. Molecular cloning and sequencing confirmed that the new DRB1 allele is identical to DRB1*0101 at exon 2 except for a single nucleotide substitution at codon 37 (ΤCC→ΤΑC), changing the encoded serine to tyrosine. This position of the β1 domain lies in the floor of the antigen‐binding groove and shows the highest polymorphism among DRB1 alleles.