Therapeutic efficacy of granulocyte colony stimulating factor against rat cecal ligation and puncture model

• Published in: Stem cells (Dayton, Ohio) Vol. 11; no. 3; p. 228
• Main Authors: Toda, H, Murata, A, Matsuura, N, Uda, K, Oka, Y, Tanaka, N, Mori, T
• Format: Journal Article
• Language: English
• Published: United States 1993
• Subjects: Animals; Bacteremia - etiology; Bacteremia - immunology; Bacteremia - therapy; Cecum; Disease Models, Animal; Granulocyte Colony-Stimulating Factor - therapeutic use; Humans; Male; Neutrophils - immunology; Peritonitis - etiology; Peritonitis - immunology; Peritonitis - therapy; Rats; Rats, Wistar; Recombinant Proteins - therapeutic use; Survival Rate
• ISSN: 1066-5099
• DOI: 10.1002/stem.5530110310

To identify the therapeutic efficacy of granulocyte colony stimulating factor (G-CSF) in severe sepsis, we examined its effect on the mortality and pathological changes in vital organs using the rat lethal sepsis model. Rats were given 15 micrograms of recombinant human (rh)G-CSF after the onset of peritonitis brought about by cecal ligation and puncture. The mortality rate after 72 h was significantly decreased by administration of 15 micrograms of rhG-CSF (p < 0.001). In addition, the administration of rhG-CSF induced an improvement in liver and renal functions. It also produced marked pathological improvement in the lungs. These results strongly indicated that administration of rhG-CSF, even after the onset of sepsis, was effective in decreasing the mortality from peritonitis-induced multiple organ failure, and this finding was clearly useful in the clinical treatment of such sepsis-induced critical illness.