Molecular and Phenotypic Characterization of Escherichia coli Associated with Granulomatous Colitis of Boxer Dogs

• Published in: Antibiotics (Basel) Vol. 9; no. 9; p. 540
• Main Authors: Dogan, Belgin, Zhang, Shiying, Kalla, Sarah E, Dogan, Esra I, Guo, Cindy, Ang, Chelston R, Simpson, Kenneth W
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.08.2020; MDPI
• Subjects: adherent-invasive E. coli (AIEC); Antigens; Autophagy; Boxer dogs; Causes of; Colitis; Cytotoxicity; Dog diseases; Dogs; E coli; Escherichia coli; fluoroquinolone; Genes; Genetic aspects; Genotype & phenotype; Genotypes; granulomatous colitis; Haplotypes; Identification and classification; Inflammation; Inflammatory bowel disease; Macrophages; monocyte derived macrophages; Monocytes; Motility; Mutation; Phylogeny; Physiological aspects; Pili; Strains (organisms); Virulence; United States; granulomatous colitis; fluoroquinolone; CD48/SLAM; Escherichia coli; Boxer dogs; monocyte derived macrophages; adherent-invasive E. coli (AIEC); very early onset inflammatory bowel disease (IBD); Crohn’s disease
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics9090540

Invasive is causally associated with granulomatous colitis (GC) of Boxer dogs and French Bulldogs. The virulence determinants of GC are unclear. isolated from 16 GC (36 strains) and 17 healthy control (HC: 33 strains) dogs were diverse in phylogeny, genotype, and serotype and lacked diarrheagenic genes. Genes encoding type II ( ), IV ( ), and VI ( ) secretion systems, long polar fimbriae ( 154/141), and iron acquisition ( , ) were frequent in GC and HC. from 14/15 GC and 10/11 HC invaded Caco-2 better than non-pathogenic strain DH5α, with invasion correlated with motility and presence of and . from all GC and 10/11 HC survived better than DH5α in J774 macrophages, with adherent-invasive (AIEC) in 60% GC and 73% HC. AIEC replicated in monocyte derived macrophages from a GC Boxer with CD48/SLAM risk haplotype but not the HC. Fluroquinolone resistant were less motile and invasive than fluoroquinolone sensitive ( < 0.05), and only 1/8 resistant strains met criteria for AIEC. In conclusion GC are diverse, resemble extraintestinal pathogenic (ExPEC), including AIEC, and can replicate in GC-susceptible macrophages. They are likely resident pathosymbionts that can opportunistically persist within macrophages of a GC-susceptible dog.