Effective Lactobacillus plantarum and Bifidobacterium infantis encapsulation with chia seed (Salvia hispanica L.) and flaxseed (Linum usitatissimum L.) mucilage and soluble protein by spray drying

• Published in: Food chemistry Vol. 216; pp. 97 - 105
• Main Authors: Bustamante, Mariela, Oomah, B. Dave, Rubilar, Mónica, Shene, Carolina
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2017
• Subjects: Bifidobacterium longum subspecies infantis - drug effects; Bifidobacterium longum subspecies infantis - growth & development; Chia seeds; Desiccation; Flax - chemistry; Flaxseed; Freeze Drying; Gastric Juice - drug effects; Gastric Juice - microbiology; Lactobacillus plantarum - drug effects; Lactobacillus plantarum - growth & development; Microencapsulation; Plant Exudates - chemistry; Powders - chemistry; Probiotics; Probiotics - administration & dosage; Salvia - chemistry; Seeds - chemistry; Spray drying; CSP; FSP; Chia seeds; Flaxseed; FM; Spray drying; CM; M; Probiotics; MD; SEM; Microencapsulation; SP
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2016.08.019

•Fractions of chia seeds (C) and flaxseed (F) could be used as encapsulating agents.•C fractions protect Lactobacillus plantarum during spray drying.•F fractions protect Bifidobacterium infantis during spray drying.•F and C fractions protect to probiotics against simulated gastrointestinal conditions. Mucilage (M) and soluble protein (SP) extracted from chia seed and flaxseed were used as encapsulating material for two probiotic bacteria: Bifidobacterium infantis and Lactobacillus plantarum by spray drying. Probiotic survival and viability after spray drying and during storage were evaluated. B. infantis and L. plantarum displayed high survival (⩾98%) after encapsulation with mixtures of maltodextrin (MD) combined with M and SP from flaxseed (MD:FM:FSP – 7.5:0.2:7.5%, w/w/w) and chia seed (MD:CM:CSP – 7.5:0.6:7.5%, w/w/w), respectively. These ternary blends protected the probiotics and enhanced their resistance to simulated gastric juice and bile solution. Probiotics encapsulated with the ternary blends incorporated in instant juice powder exhibited high viability (>9Log10CFU/g) after 45days refrigerated storage. Encapsulation with the ternary blends reduced particle size of the probiotic powders thereby offering additional functional benefits. Our results reveal that chia seed and flaxseed are excellent sources of probiotic encapsulating agents.