Compensation of aberration in quantitative phase imaging using lateral shifting and spiral phase integration

We present a simple and effective method to eliminate system aberrations in quantitative phase imaging. Using spiral phase integration, complete information about system aberration is calculated from three laterally shifted phase images. The present method is especially useful when measuring conflue...

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Bibliographic Details
Published inOptics express Vol. 25; no. 24; pp. 30771 - 30779
Main Authors Choi, Inhyeok, Lee, KyeoReh, Park, YongKeun
Format Journal Article
LanguageEnglish
Published United States 27.11.2017
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Summary:We present a simple and effective method to eliminate system aberrations in quantitative phase imaging. Using spiral phase integration, complete information about system aberration is calculated from three laterally shifted phase images. The present method is especially useful when measuring confluent samples in which acquisition of background area is challenging. To demonstrate validity and applicability, we present measurements of various types of samples including microspheres, HeLa cells, and mouse brain tissue. Working conditions and limitations are systematically analyzed and discussed.
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ISSN:1094-4087
1094-4087
DOI:10.1364/oe.25.030771