Effects of dissolved organic material on binding and toxicokinetics of pyrene in the waterflea Daphnia magna

• Published in: Archives of environmental contamination and toxicology Vol. 40; no. 3; pp. 333 - 338
• Main Authors: NIKKILÄ, A, KUKKONEN, J. V. K
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.04.2001; Berlin Springer Nature B.V; New York, NY
• Subjects: Animal, plant and microbial ecology; Animals; Applied ecology; Bioavailability; Biological and medical sciences; Biological Availability; Daphnia; Daphnia magna; dissolved organic matter; Ecotoxicology, biological effects of pollution; Effects of pollution and side effects of pesticides on protozoa and invertebrates; Finland; Finland, Kontiolampi L; Finland, Pohjois-Karjala, Pielisjoki R; Fluorescent Dyes - chemistry; Fluorescent Dyes - pharmacokinetics; Fluorescent Dyes - toxicity; Fresh water environment; Freshwater; Fundamental and applied biological sciences. Psychology; Organic Chemicals; pyrene; Pyrenes - chemistry; Pyrenes - pharmacokinetics; Pyrenes - toxicity; Solubility; Tissue Distribution; Water Pollutants, Chemical - pharmacokinetics; Water Pollutants, Chemical - toxicity; Finland, Itae-Suomi, Kontiolampi L; Finland, Itae-Suomi, Pielisjoki R; Organic matter; Cladocera; Elimination; Environmental factor; Partition coefficient; Bioavailability; Auroral zone; Polycyclic aromatic compound; Spatial variation; Crustacea; Freshwater environment; Dissolved organic carbon; Toxicokinetics; Pyrene; Binding capacity; Humus; Arthropoda; Lakes; Water pollution; Stream; Invertebrata; Biological accumulation; Branchiopoda; Daphnia magna
• ISSN: 0090-4341; 1432-0703
• DOI: 10.1007/s002440010180

The binding and bioavailability of pyrene was studied in the laboratory in two humic fresh waters and in a reference water without dissolved organic material (DOM), measured as dissolved organic carbon (DOC). The uptake of pyrene by Daphnia magna in short-term (24 h) accumulation experiments was fitted to a first-order rate-kinetic equation to calculate simultaneous uptake and elimination rates. The partition coefficients of pyrene to DOC (KDOC) were 37.1 x 103 in Pielisjoki River (9.4 mg DOC L(-1)), and 34.9 x 103 in Lake Kontiolampi (17.4 mg DOC L(-1)) waters, indicating similar binding affinities of pyrene for both humic waters. The uptake clearance of pyrene (ku) in the DOC-rich Lake Kontiolampi water was 290.4 ml g wet weight(-1) h(-1), which was lower than those in Pielisjoki River and DOC-free waters (395.8 and 346.0 ml g wet weight(-1) h(-1), respectively). Pyrene elimination (ke) was in average 0.2 h(-1) with no significant differences between the waters. The observed bioconcentration factors of pyrene in Pielisjoki River and Lake Kontiolampi waters were 84% and 46% of that in the DOC-free reference water, respectively, thus indicating that a high concentration of DOC is needed to decrease the bioavailability of waterborne pyrene to D. magna.