Metagenomic analysis of the bacterial microbiota associated with cultured oysters (Crassostrea sp.) in estuarine environments

Abstract In this work, we identified the bacterial microbiota associated with farmed oystersin estuarine regions of four states in the north eastern region of Brazil. During the drought and rainy seasons, for eight months, twenty oysters were sampled seasonally from seven different marine farms. In...

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Published inAnais da Academia Brasileira de Ciências Vol. 92; no. suppl 1; p. e20180432
Main Authors HORODESKY, ALINE, CASTILHO-WESTPHAL, GISELA G., PONT, GIORGI DAL, FAORO, HELISSON, BALSANELLI, EDUARDO, TADRA-SFEIR, MICHELLE Z., COZER, NATHIELI, PIE, MARCIO ROBERTO, OSTRENSKY, ANTONIO
Format Journal Article
LanguageEnglish
Portuguese
Published Academia Brasileira de Ciências 2020
Subjects
contamination
cultivation
MULTIDISCIPLINARY SCIENCES
oyster farming
pathogenicity
pathogenicity
cultivation
oyster farming
contamination
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Summary:Abstract In this work, we identified the bacterial microbiota associated with farmed oystersin estuarine regions of four states in the north eastern region of Brazil. During the drought and rainy seasons, for eight months, twenty oysters were sampled seasonally from seven different marine farms. In the laboratory, DNA extraction, amplification, and sequencing of the 16S rRNA gene were performed to establish the taxonomic units. We identified 106 genera of bacteria belonging to 103 families, 70 orders, 39 classes, and 21 phyla. Out of the total, 40 of the genera represented bacteria potentially pathogenic to humans; of these, nine are known to cause foodborne diseases and six are potentially pathogenic to oysters. The most prevalent genera were Mycoplasma, Propionigenium, Psychrilyobacter, and Arcobacter. The results indicate the need for more systematic monitoring of bacteria of the genus Mycoplasma in oyster farming operations in the Brazilian north eastern region. Currently, Mycoplasma is not one of the microorganisms analysed and monitored by order of Brazilian legislation during the oyster production and/or commercialization process, even though this genus was the most prevalent at all sampling points and presents pathogenic potential both for oysters and for consumers.
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ISSN:0001-3765
1678-2690
1678-2690
DOI:10.1590/0001-3765202020180432