Curcumin derivative, C817 inhibits proliferation of imatinib-resistant chronic myeloid leukemia cells with wild-type or mutant Bcr-Abl in vitro

• Published in: Acta pharmacologica Sinica Vol. 35; no. 3; pp. 401 - 409
• Main Authors: Wu, Li-xian, Wu, Ying, Chen, Rui-jia, Liu, Yang, Huang, Li-sen, Lou, Li-guang, Zheng, Zhi-hong, Chen, Yuan-zhong, Xu, Jian-hua
• Format: Journal Article
• Language: English
• Published: United States Nature Publishing Group 01.03.2014
• Subjects: Antineoplastic Agents, Phytogenic - pharmacology; Apoptosis - drug effects; Benzamides - pharmacology; Cell Proliferation - drug effects; Curcumin - analogs & derivatives; Curcumin - pharmacology; Dose-Response Relationship, Drug; Drug Resistance, Neoplasm - genetics; Fusion Proteins, bcr-abl - antagonists & inhibitors; Fusion Proteins, bcr-abl - genetics; Fusion Proteins, bcr-abl - metabolism; Genetic Predisposition to Disease; Humans; Imatinib Mesylate; K562 Cells; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - enzymology; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - pathology; Mutation; Neoplastic Stem Cells - drug effects; Neoplastic Stem Cells - enzymology; Neoplastic Stem Cells - pathology; Original; Phenotype; Phosphorylation; Piperazines - pharmacology; Protein Kinase Inhibitors - pharmacology; Pyrimidines - pharmacology; 姜黄素; 慢性粒细胞白血病; 白血病细胞; 突变型; 细胞增殖; 耐药; 衍生物; 野生型
• ISSN: 1671-4083; 1745-7254
• DOI: 10.1038/aps.2013.180

Aim： To find new kinase inhibitors that overcome time imatinib resistance in treatment of chronic myeloid leukemia （CML）, we synthesized C817, a novel derivative of curcumin, and tested its activities against wild-type （WT） and imatinib-resistant mutant Abl kinases, as well as in imatinib-sensitive and resistant CML cells in vitro. Methods： 32D cells harboring WT or mutant Abl kinases （nucleotide binding P-loop mutants Q252H, Y253F, and imatinib contact residue mutant T3151）, as well as K562/G01 cells （with whole Bcr-Abl gene amplication） were tested. Kinase activity was measured using Kinase-GIo Luminescent Kinase Assay Platform in recombinant WT and mutant （Q252H, Y253F, and T3151） Abl kinases. Cell proliferation and apoptosis were examined using MTT assay and flow cytometry, respectively. The phosphorylation levels of Bcr-Abl initiated signaling proteins were analyzed using Western blotting. Colony forming units （CFU） growth and long term culture-initiating cells （LTC-ICs） were used to test the effects of C817 on human leukemia progenitor/stem cells. Results： C817 potently inhibited both WT and mutant （Q252H, Y253F, and T3151） Abl kinase activities in a non-ATP competitive manner with the values of ICso at low nanomole levels. In consistent with above results, C817 suppressed the growth of both imatinib-sensitive and resistant CML cells, including wild-type K562, K562/GO$, 32D-T3151, 32D-Q252H, and 32D-Y253F cells with the values of ICso at low micromole levels. C817 （0.5 or 1 tJmol/L） dose-dependently inhibited the phosphorylation of Bcr-Abl and downstream proteins STAT-5 and CrkL in imatinib-resistant K562/G01 cells. Furthermore, C817 significantly suppressed CFU growth and LTC-ICs, implicating that C817 could eradiate human leukemia progenitor/stem cells. Conclusion： C817 is a promising compound for treatment of CML patients with Bcr-Abl kinase domain mutations that confer imatinib resistance.