Ginkgo biloba extract EGb761 attenuates brain death-induced renal injury by inhibiting pro-inflammatory cytokines and the SAPK and JAK-STAT signalings

• Published in: Scientific reports Vol. 7; no. 1; p. 45192
• Main Authors: Li, Yifu, Xiong, Yunyi, Zhang, Huanxi, Li, Jun, Wang, Dong, Chen, Wenfang, Yuan, Xiaopeng, Su, Qiao, Li, Wenwen, Huang, Huiting, Bi, Zirong, Liu, Longshan, Wang, Changxi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23.03.2017; Nature Publishing Group
• Subjects: 14/28; 38/77; 692/308/409; 692/4022/1950/2777; Acute Kidney Injury - drug therapy; Acute Kidney Injury - etiology; Acute Kidney Injury - prevention & control; Animals; Anti-Inflammatory Agents - therapeutic use; Brain death; Brain Death - blood; Brain Death - metabolism; Cytokines; Cytokines - genetics; Cytokines - metabolism; Humanities and Social Sciences; IL-1β; Inflammation; JNK protein; Kidney - metabolism; Kidney - pathology; Kidney Transplantation - adverse effects; Macrophages; Male; MAP Kinase Signaling System; multidisciplinary; Plant Extracts - therapeutic use; Rats; Rats, Sprague-Dawley; Rodents; Science; STAT Transcription Factors - metabolism; Tissue Donors; Transplants - pathology; Transplants - standards
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep45192

This study aimed to investigate the protective effects of EGb761, a Ginkgo Biloba extract, against brain death-induced kidney injury. Sixty male Sprague Dawley rats were randomly divided into six groups: sham, brain-death (BD), BD + EGb b48h (48 hours before BD), BD + EGb 2 h (2 hours after BD), BD + EGb 1 h, and BD + EGb 0.5 h. Six hours after BD, serum sample and kidney tissues were collected for analyses. The levels of blood urea nitrogen (BUN) and serum creatinine significantly elevated in the BD group than in sham group. In all the EGb761-treated BD animals except for the BD + Gb 2 h group, the levels of BUN and serum creatinine significantly reduced (all P  < 0.01). EGb761 attenuated tubular injury and lowered the histological score. In addition, the longer duration of drug treatment was, the better protective efficacy could be observed. EGb761 significantly reduced IL-1β, IL-6, TNF-α, MCP-1, IP-10 mRNA expression and macrophage infiltration in the kidney. EGb761 treatment at 48 hour before brain death significantly attenuate the levels of p-JNK-MAPK, p-p38-MAPK, and p-STAT3 proteins (all P < 0.05, compared to BD group). In summary, our data showed that EGb761 treatment protected donor kidney from BD-induced damages by blocking SAPK and JAK-STAT signalings. Early administration of EGb761 can provide better protective efficacy.