The complex nature of calcium cation interactions with phospholipid bilayers

• Published in: Scientific reports Vol. 6; no. 1; p. 38035
• Main Authors: Melcrová, Adéla, Pokorna, Sarka, Pullanchery, Saranya, Kohagen, Miriam, Jurkiewicz, Piotr, Hof, Martin, Jungwirth, Pavel, Cremer, Paul S., Cwiklik, Lukasz
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.12.2016; Nature Publishing Group
• Subjects: 119/118; 14/34; 631/57/2270; 631/57/2272; 639/638/92/56; Binding sites; Calcium; Calcium buffering; Calcium signalling; Calcium-binding protein; Humanities and Social Sciences; Light scattering; Lipid membranes; Lipid monolayers; Lipids; Membrane fusion; Membrane proteins; Membranes; multidisciplinary; Phospholipids; Polarization; Science; Spectroscopy; Spectrum analysis; Synaptic plasticity; Vesicles; Zeta potential
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep38035

Understanding interactions of calcium with lipid membranes at the molecular level is of great importance in light of their involvement in calcium signaling, association of proteins with cellular membranes, and membrane fusion. We quantify these interactions in detail by employing a combination of spectroscopic methods with atomistic molecular dynamics simulations. Namely, time-resolved fluorescent spectroscopy of lipid vesicles and vibrational sum frequency spectroscopy of lipid monolayers are used to characterize local binding sites of calcium in zwitterionic and anionic model lipid assemblies, while dynamic light scattering and zeta potential measurements are employed for macroscopic characterization of lipid vesicles in calcium-containing environments. To gain additional atomic-level information, the experiments are complemented by molecular simulations that utilize an accurate force field for calcium ions with scaled charges effectively accounting for electronic polarization effects. We demonstrate that lipid membranes have substantial calcium-binding capacity, with several types of binding sites present. Significantly, the binding mode depends on calcium concentration with important implications for calcium buffering, synaptic plasticity, and protein-membrane association.