Polymerase chain reaction analysis of eae gene subtypes present in attaching and effacing Escherichia coli isolated from pigs with diarrhea

• Published in: Journal of veterinary diagnostic investigation Vol. 16; no. 6; pp. 576 - 578
• Main Authors: Ha, S.K, Hong, K, Choi, C.S, Jung, K, Ha, Y.C, Kim, J.H, Kim, S.H, Yoon, B.S, Chae, C.H
• Format: Journal Article
• Language: English
• Published: United States 01.11.2004
• Subjects: Adhesins, Bacterial - genetics; Animals; diarrhea; Diarrhea - microbiology; Diarrhea - veterinary; DNA Primers - genetics; enterovirulent Escherichia coli group; Escherichia coli - isolation & purification; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Escherichia coli Proteins - genetics; Escherichia infections; genes; genetic variation; Genotype; piglets; polymerase chain reaction; Polymerase Chain Reaction - veterinary; strain differences; Swine; Swine Diseases - microbiology; weaning
• ISSN: 1040-6387; 1943-4936
• DOI: 10.1177/104063870401600614

In this study the subtype of eae gene was determined by polymerase chain reaction for a total of 59 attaching and effacing Escherichia coli isolated from preweaned (38 isolates) and postweaned (21 isolates) pigs. The eae(beta) gene detected in 19 E. coli from preweaned pigs and 10 E. coli from postweaned pigs was found to be the most common subtype, followed by eae(gamma), eae(epsilon), and eae(zeta) genes. Subtypes were not determined for 7 E. coli isolates. No other subtype of the eae gene was detected in eae+ E. coli evaluated in this study.