Measurement of thiamylal and thiopental in plasma by electron capture and flame photometric gas-liquid chromatography

We describe an isothermal gas-liquid chromatographic procedure developed for measuring thiamylal and thiopental in plasma. The unchanged drug is extracted into ethyl acetate from acidified plasma, together with an internal standard. The solvent is removed, the residue methylated, aliquots, diluted w...

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Bibliographic Details
Published inClinical chemistry (Baltimore, Md.) Vol. 23; no. 7; pp. 1306 - 1309
Main Authors Smith, RH, MacDonald, JA, Thompson, DS, Flacke, WE
Format Journal Article
LanguageEnglish
Published England Am Assoc Clin Chem 01.07.1977
Subjects
Chromatography, Gas - methods
Humans
Methods
Thiamylal - blood
Thiopental - blood
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Summary:We describe an isothermal gas-liquid chromatographic procedure developed for measuring thiamylal and thiopental in plasma. The unchanged drug is extracted into ethyl acetate from acidified plasma, together with an internal standard. The solvent is removed, the residue methylated, aliquots, diluted with benzene, are injected into a 183-cm gas-liquid chromatographic column containing 3% OV-17. Sensitivity of detection is in the nanogram to picogram range.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/23.7.1306