Applications and performance of monoclonal antibodies to human tartrate resistant acid phosphatase

• Published in: Journal of immunological methods Vol. 372; no. 1; pp. 162 - 170
• Main Authors: Pradella, Silvia D. Potenziani, Slone, Stephen P., Wu, Yi-Ying, Chao, Tsu-Yi, Parthasarathy, Ranga N., Yam, Lung T., Janckila, Anthony J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 30.09.2011; Elsevier
• Subjects: acid phosphatase; Acid Phosphatase - analysis; Acid Phosphatase - blood; acid tolerance; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - immunology; autoimmune diseases; Biological and medical sciences; biomarkers; Biomarkers - blood; blood serum; Bone Diseases - diagnosis; Bone Diseases - enzymology; Dendritic cell; enzyme activity; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunoassay; Immunoassay - methods; immunoassays; immunohistochemistry; Isoenzymes - analysis; Isoenzymes - blood; Macrophage; macrophages; Molecular immunology; Monoclonal antibodies; Osteoclast; osteoclasts; precipitin tests; Sensitivity and Specificity; Tartrate-Resistant Acid Phosphatase; Techniques; Western blotting; Immunoassay; Dendritic cell; Tartrate-resistant acid phosphatase; IHC; HRP; IP; IL-4; Osteoclast; WB; SPENCD; Mab; OC; GM-CSF; TRACP; Monoclonal antibodies; MΦ; LSC; DC; Macrophage; Human; Enzyme; Phosphoric monoester hydrolases; Esterases; Monoclonal antibody; Immunological method; Resistance; Tartrate; Hydrolases; Acid phosphatase
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/j.jim.2011.07.010

Tartrate-resistant acid phosphatase (TRACP) is an enzyme common to cells of the mononuclear phagocyte system and a clinically relevant biomarker for osteoclasts and inflammatory macrophages. The purpose was to assess applications and performance of six anti-TRACP monoclonal antibodies. Mab9 C5, 14 G6, 162, 203, 220, and 89 were used as capture and detection antibodies in quantitative immunoassay, and for western blot (WB), immunoprecipitation, and immunohistochemistry of paraffin sections containing chronic inflammatory infiltrates. The clinical performance of mab14 G6 for immunoassay of serum TRACP5b activity was compared to two commercial kit methods. Mab9 C5 is useful for WB and immunohistochemistry methods only. Mab14G6, 162, and 203 are useful for quantitative immunoassay and immunoprecipitation, however, mab203 causes inactivation of enzymatic activity. Mab220 and 89 are specific for TRACP5a and useful in all applications. Mab14G6 has similar clinical sensitivity and specificity as two commercial methods. TRACP is an important marker in osteoimmunology. Specific antibodies with unique specificity for TRACP isoforms and defined applications will be valuable for clinical evaluation of bone metabolic, inflammatory and autoimmune diseases and will aid in basic research of TRACP biochemistry and biology. ► TRACP is a biomarker of mononuclear phagocyte system expressed as two isoforms, 5a and 5b, with separate clinical significance ► TRACP5a is secreted by macrophages; serum levels may indicate severity of chronic inflammatory diseases ► TRACP5b is secreted by osteoclasts in proportion to their number and systemic bone resorption rate ► Anti-TRACP antibodies have selective applications and unique isoform specificities in quantitative immunoassay, western blot, immunoprecipitation and immunohistochemistry