Selecting an indigenous microalgal strain for lipid production in anaerobically treated piggery wastewater

• Published in: Bioresource technology Vol. 191; pp. 369 - 376
• Main Authors: Marjakangas, Jatta M., Chen, Chun-Yen, Lakaniemi, Aino-Maija, Puhakka, Jaakko A., Whang, Liang-Ming, Chang, Jo-Shu
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2015
• Subjects: Anaerobiosis; Animals; Chlorella vulgaris; Lipid production; Lipids - biosynthesis; Microalgae - metabolism; Piggery wastewater; Swine; Waste Water; Water Purification; Lipid production; Chlorella vulgaris; Piggery wastewater
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2015.02.075

•Biomass and lipid production ability of three microalgal strains was studied.•High lipid content was achieved with C. vulgaris in pre-treated piggery wastewater.•C. vulgaris in piggery wastewater excreted xylose, mannose, and arabinose. The aim of this study was to select a potential microalgal strain for lipid production and to examine the suitability of anaerobically treated piggery wastewater as a nutrient source for production of lipid-rich biomass with the selected microalga. Biomass and lipid productivity of three microalgal strains (Chlorella sorokiniana CY1, Chlorella vulgaris CY5 and Chlamydomonas sp. JSC-04) were compared by using different media, nitrogen sources, and nitrogen concentrations. The highest lipid content and productivity (62.5wt%, 162mg/L/d) were obtained with C. vulgaris with BG-11 with 62mgN/L. Secondly, C. vulgaris was cultivated in sterilized, diluted (1–20×), anaerobically treated piggery wastewater. Biomass production decreased and lipid content increased, when wastewater was more diluted. The highest lipid content of 54.7wt% was obtained with 20× dilution, while the highest lipid productivity of 100.7mg/L/d with 5× dilution. Piggery wastewater is a promising resource for mass production of oleaginous microalgal biomass.