A microfluidic device for efficient chemical testing using Caenorhabditis elegans

The nematode worm Caenorhabditis elegans has been employed as a popular model organism in many fields of biological research. In this paper, we present a microfluidic device for facilitating chemical testing using C. elegans . For testing chemicals on chip, the device houses single nematodes in micr...

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Bibliographic Details
Published inBiomedical microdevices Vol. 17; no. 2; pp. 38 - 10
Main Authors Song, Pengfei, Zhang, Weize, Sobolevski, Alexandre, Bernard, Kristine, Hekimi, Siegfried, Liu, Xinyu
Format Journal Article
LanguageEnglish
Published New York Springer US 01.04.2015
Springer Nature B.V
Subjects
Animals
Biological and Medical Physics
Biomedical Engineering and Bioengineering
Biophysics
Caenorhabditis elegans
Caenorhabditis elegans - drug effects
Caenorhabditis elegans - physiology
Chambers
Channels
Computer programs
Devices
Engineering
Engineering Fluid Dynamics
Equipment Design
Glucose - pharmacology
Houses
Lab-On-A-Chip Devices
Locomotion
Microfluidic Analytical Techniques - instrumentation
Microfluidics
Nanotechnology
Nematoda
Nematodes
Quantitative analysis
Software
Stimulation, Chemical
User-Computer Interface
Zinc
Zinc - pharmacology
Locomotion
Behavior studies
Chemical testing
Image processing
Microfluidics
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Summary:The nematode worm Caenorhabditis elegans has been employed as a popular model organism in many fields of biological research. In this paper, we present a microfluidic device for facilitating chemical testing using C. elegans . For testing chemicals on chip, the device houses single nematodes in microfluidic chambers and precisely adjusts the chamber’s chemical environment during experiments. Eight nematodes can be readily loaded into the chambers through separate loading channels in a quick and gentle manner. In addition, a custom-made software with a graphic user interface is also created for quantitative analysis of locomotion parameters (swimming frequency and bend amplitude) of the nematodes in response to chemical stimuli, thus greatly enhancing the efficiency of data collection. We perform proof-of-concept experiments using two chemicals, zinc ion (Zn 2+ ) and glucose, to demonstrate the effectiveness of the microfluidic device.
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ISSN:1387-2176
1572-8781
DOI:10.1007/s10544-015-9939-8