Advancing Microfluidic Immunity Testing Systems: New Trends for Microbial Pathogen Detection

Pathogenic microorganisms play a crucial role in the global disease burden due to their ability to cause various diseases and spread through multiple transmission routes. Immunity tests identify antigens related to these pathogens, thereby confirming past infections and monitoring the host’s immune...

Full description

Saved in:
Bibliographic Details
Published inMolecules (Basel, Switzerland) Vol. 29; no. 14; p. 3322
Main Authors Wang, Yiran, Chen, Jingwei, Zhang, Yule, Yang, Zhijin, Zhang, Kaihuan, Zhang, Dawei, Zheng, Lulu
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 15.07.2024
Subjects
3-D printers
Accuracy
Antibodies
Automation
Blood tests
COVID-19
Dengue fever
Disease transmission
Environmental monitoring
high throughput
Immunoassay
Influenza
Laboratories
Manufacturing
microbial pathogen
microfluidic
Microorganisms
Pathogens
Public health
rapid diagnosis
Reagents
Screen printing
Serology
Severe acute respiratory syndrome coronavirus 2
Viruses
microbial pathogen
microfluidic
high throughput
rapid diagnosis
immunoassay
Online AccessGet full text

Cover

More Information
Summary:Pathogenic microorganisms play a crucial role in the global disease burden due to their ability to cause various diseases and spread through multiple transmission routes. Immunity tests identify antigens related to these pathogens, thereby confirming past infections and monitoring the host’s immune response. Traditional pathogen detection methods, including enzyme-linked immunosorbent assays (ELISAs) and chemiluminescent immunoassays (CLIAs), are often labor-intensive, slow, and reliant on sophisticated equipment and skilled personnel, which can be limiting in resource-poor settings. In contrast, the development of microfluidic technologies presents a promising alternative, offering automation, miniaturization, and cost efficiency. These advanced methods are poised to replace traditional assays by streamlining processes and enabling rapid, high-throughput immunity testing for pathogens. This review highlights the latest advancements in microfluidic systems designed for rapid and high-throughput immunity testing, incorporating immunosensors, single molecule arrays (Simoas), a lateral flow assay (LFA), and smartphone integration. It focuses on key pathogenic microorganisms such as SARS-CoV-2, influenza, and the ZIKA virus (ZIKV). Additionally, the review discusses the challenges, commercialization prospects, and future directions to advance microfluidic systems for infectious disease detection.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ObjectType-Review-3
content type line 23
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules29143322