Influence of GSTM1 and GSTT1 genotypes and confounding factors on the frequency of sister chromatid exchange and micronucleus among road construction workers

• Published in: Chemosphere (Oxford) Vol. 84; no. 5; pp. 564 - 570
• Main Authors: Kumar, Anil, Yadav, Anita, Giri, Shiv Kumar, Dev, Kapil, Gautam, Sanjeev Kumar, Gupta, Ranjan, Aggarwal, Neeraj
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.07.2011; Elsevier
• Subjects: Adult; Biological and medical sciences; Chemical and industrial products toxicology. Toxic occupational diseases; Cytokinesis blocked micronuclei; Environmental Pollutants - toxicity; Female; Gene Frequency; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Glutathione-S transferase; High frequency cell; Humans; Leukocytes, Mononuclear - metabolism; Male; Medical sciences; Micronuclei, Chromosome-Defective; Middle Aged; Occupational Exposure - statistics & numerical data; Sister Chromatid Exchange; Toxicology; Various organic compounds; Young Adult; Glutathione-S transferase; High frequency cell; Cytokinesis blocked micronuclei; Sister chromatid exchange; Hydrocarbon; Enzyme; Toxicity; Transferases; Road construction; Genotype; Exchange; Pollutant; Glutathione transferase; Micronucleus; Chromatid; Worker
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2011.04.008

► Road construction workers showed higher cytogenetic damage in PBL. ► GST +ve genotype was found more prevalent among studied populations. ► Positive interaction was found between GST genotypes, confounding factors and cytogenetic biomarkers. ► Individuals having null genotypes of GSTM1 and GSTT1 had higher cytogenetic damage. In the present study, we have investigated the influence of polymorphism of GSTM1 and GSTT1 genes and confounding factors such as age, sex, exposure duration and consumption habits on cytogenetic biomarkers. Frequency of sister chromatid exchanges (SCEs), high frequency cell (HFC) and cytokinesis blocked micronuclei (CBMN) were evaluated in peripheral blood lymphocytes of 115 occupationally exposed road construction workers and 105 unexposed individuals. The distribution of null and positive genotypes of glutathione-S transferase gene was evaluated by multiplex PCR among control and exposed subjects. An increased frequency of CBMN (7.03 ± 2.08); SCE (6.95 ± 1.76) and HFC (6.28 ± 1.69) were found in exposed subjects when compared to referent (CBMN – 3.35 ± 1.10; SCE – 4.13 ± 1.30 and HFC – 3.98 ± 1.56). These results were found statistically significant at p < 0.05. When the effect of confounding factors on the frequency of studied biomarkers was evaluated, a strong positive interaction was found. The individuals having GSTM1 and GSTT1 null genotypes had higher frequency of CBMN, SCE and HFC. The association between GSTM1 and GSTT1 genotypes and studied biomarkers was found statistically significant at p < 0.05. Our findings suggest that individuals having null type of GST are more susceptible to cytogenetic damage by occupational exposure regardless of confounding factors. There is a significant effect of polymorphism of these genes on cytogenetic biomarkers which are considered as early effects of genotoxic carcinogens.