Increased plasma levels of soluble CD40 ligand correlate with platelet activation markers and underline the need for standardized pre-analytical conditions
To investigate whether sCD40L dosage might represent a useful tool to explore in vivo platelet function. sCD40L and sP-selectin levels and light transmission aggregometry (LTA) were analyzed in 69 healthy donors. Immunoassays were performed on platelet-depleted citrate plasma samples. The effects of...
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Published in | Clinical biochemistry Vol. 43; no. 7; pp. 666 - 670 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier Inc
01.05.2010
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | To investigate whether sCD40L dosage might represent a useful tool to explore in vivo platelet function.
sCD40L and sP-selectin levels and light transmission aggregometry (LTA) were analyzed in 69 healthy donors. Immunoassays were performed on platelet-depleted citrate plasma samples. The effects of in vitro aspirin treatment on the release of sCD40L were investigated in 15 subjects following platelet stimulation. The effects of a 1-month therapeutic course of low-dose aspirin on sP-selectin and sCD40L levels were also investigated.
A significant correlation was observed between sCD40L and sP-selectin (
p
<
0.01).
In vitro aspirin treatment remarkably decreased sCD40L levels following platelet activation by exogenous agonists. sCD40L directly correlated with LTA (Rho
=
0.62,
p
<
0.0001). In vivo aspirin treatment significantly reduced both sP-selectin and sCD40L levels (both
p
<
0.01) in a direct correlation (Rho
=
0.66,
p
<
0.05).
Citrated plasma samples reflect sCD40L released from platelets, thus yielding the most valid estimates of in vivo circulating levels of this platelet activation markers. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0009-9120 1873-2933 |
DOI: | 10.1016/j.clinbiochem.2009.12.021 |