Overexpression of DHX32 contributes to the growth and metastasis of colorectal cancer

• Published in: Scientific reports Vol. 5; no. 1; p. 9247
• Main Authors: Lin, Huayue, Liu, Wenjuan, Fang, Zanxi, Liang, Xianming, Li, Juan, Bai, Yongying, Lin, Lingqing, You, Hanyu, Pei, Yihua, Wang, Fen, Zhang, Zhong-Ying
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.03.2015; Nature Publishing Group
• Subjects: 13/1; 13/109; 13/2; 13/31; 13/89; 13/95; 14/63; 38/61; 5-Fluorouracil; 631/67/395; 631/80/86; Antigens, Neoplasm - metabolism; Antineoplastic Agents - pharmacology; Apoptosis; Apoptosis - drug effects; Carbonic Anhydrase IX; Carbonic Anhydrases - metabolism; Cell Line, Tumor; Cell Movement - drug effects; Cell proliferation; Cell Proliferation - drug effects; Chemotherapy; Coenzyme A Ligases - metabolism; Colon cancer; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; DEAD-box RNA Helicases - antagonists & inhibitors; DEAD-box RNA Helicases - genetics; DEAD-box RNA Helicases - metabolism; Fluorouracil - pharmacology; Humanities and Social Sciences; Humans; Matrilysin; Metastases; Metastasis; multidisciplinary; Polymerase chain reaction; Proto-Oncogene Proteins c-bcr - metabolism; RNA Interference; RNA, Small Interfering - metabolism; Science; Up-Regulation - drug effects; Wnt protein; Wnt Signaling Pathway - drug effects
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep09247

Our previous work demonstrates that DHX32 is upregulated in colorectal cancer (CRC) compared to its adjacent normal tissues. However, how overexpressed DHX32 contributes to CRC remains largely unknown. In this study, we reported that DHX32 was overexpressed in human colon cancer cells. Overexpressed DHX32 promoted SW480 cancer cells proliferation, migration and invasion, as well as decreased the susceptibility to chemotherapy agent 5-Fluorouracil. Furthermore, PCR array analyses revealed that depleting DHX32 in SW480 colon cancer cells suppressed expression of WISP1 , MMP7 and VEGFA in the Wnt pathway and anti-apoptotic gene BCL2 and CA9 , however, elevated expression of pro-apoptotic gene ACSL5. The findings suggested that overexpressed DHX32 played an important role in CRC progression and metastasis and that DHX32 has the potential to serve as a biomarker and a novel therapeutic target for CRC.