Effect of hydrogen peroxide on somatic embryogenesis of Lycium barbarum L

• Published in: Plant science (Limerick) Vol. 146; no. 1; pp. 9 - 16
• Main Authors: Kairong, Cui, Gengsheng, Xing, Xinmin, Liu, Gengmei, Xing, Yafu, Wang
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 13.08.1999; Elsevier Science
• Subjects: application rate; Biological and medical sciences; Biotechnology; callus; Catalase; cell division; culture media; embryo (plant); embryogenesis; enzyme activity; enzyme inhibitors; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Hydrogen peroxide; In vitro propagation: entire plant regeneration from tissues and cell cultures; Lycium; Lycium barbarum L; Methods. Procedures. Technologies; Peroxidase; Plant cells and fungal cells; SOD; Somatic embryogenesis; superoxide dismutase; SOD; Peroxidase; Hydrogen peroxide; Catalase; Lycium barbarum L; Somatic embryogenesis; Embryonic development; Enzyme; Superoxide dismutase; Micropropagation; Oxidant; Cell differentiation; Hydrogen Peroxides; Oxygen ion; Free radical; Medicinal plant; Somatic embryo; Enzymatic activity; Peroxidases; Dicotyledones; Angiospermae; Spermatophyta; Application method; Oxidoreductases; Solanaceae
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(99)00087-4

The subject of this study is inducing somatic embryogenesis in the callus of Lycium barbarum L. and determining hydrogen peroxide in somatic embryogenesis. First of all, the activities of three antioxidant enzymes (SOD, peroxidase, catalase) in different stages of somatic embryogenesis were determined. The result showed that the activity of SOD gradually increased in the early days of differentiation culture and gradually decreased along with further division and development of multi-cellular embryos. The peak of SOD activity arose on the seventh day of culture, that was exactly the time that multi-cellular embryos were formed by embryogenic cell division. Peroxidase and catalase activities were high in callus, and rapidly decreased in the early days of the differentiation culture. Aminotriazole (AT) as the inhibitor of catalase inhibited catalase activity by 85% and increased the frequency of somatic embryogenesis. Diethyldithiocarbonate (DDC) as the inhibitor of SOD inhibited SOD activity by 80% and decreased the frequency of somatic embryogenesis. Therefore, we suggest that there is an increase of intracellular H 2O 2 in the formation of embryogenic cells. Secondly, the effect of different concentrations of exogenous hydrogen peroxide (H 2O 2) in somatic embryogenesis were determined. The frequency of somatic embryogenesis reached its maximal value after treatment with 200 μM exogenous H 2O 2 for 15 days. Higher concentrations of H 2O 2 (300 μM) inhibited the formation of somatic embryos. In addition, the intracellular levels of H 2O 2 were also determined in somatic embryogenesis. Intracellular levels of H 2O 2 were higher in the formation of embryogenic cell than in callus. The results showed that the differentiation of embryogenic cells was affected by H 2O 2. Higher levels of intracellular H 2O 2 induced and promoted somatic embryogenesis. Finally, the study also discusses the effect of oxidative stress in cell differentiation and somatic embryogenesis.