Seasonal dynamics of the microbial community in two full-scale wastewater treatment plants: Diversity, composition, phylogenetic group based assembly and co-occurrence pattern

• Published in: Water research (Oxford) Vol. 200; p. 117295
• Main Authors: Sun, Chenxiang, Zhang, Bing, Ning, Daliang, Zhang, Ya, Dai, Tianjiao, Wu, Linwei, Li, Tianle, Liu, Wei, Zhou, Jizhong, Wen, Xianghua
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 15.07.2021
• Subjects: activated sludge; Assembly; autumn; data collection; microbial communities; Microbial community; nitrification; phylogeny; Seasonal dynamic; summer; wastewater treatment; Wastewater treatment plant; water; winter; Wastewater treatment plant; Microbial community; Assembly; Seasonal dynamic
• ISSN: 0043-1354; 1879-2448; 1879-2448
• DOI: 10.1016/j.watres.2021.117295

lPhylogenetic group based AS assembly is identified by long time data of two WWTPs.lThe assembly of one third (relative abundance) AS community is deterministic-dominated.lThe microbial diversity pattern is more stable in AS than influent community.lThe composition of AS community has a seasonal succession pattern.lThe network structure of AS community is more stable in summer and autumn. The optimal operation and functional stability of a wastewater treatment plant (WWTP) strongly depend on the properties of its microbial community. However, a knowledge gap remains regarding the seasonal dynamics of microbial community properties, especially phylogenetic group based assembly and co-occurrence patterns. Accordingly, in this study, influent and activated sludge (AS) samples were weekly collected from 2 full-scale WWTPs for one year (89 influent and 103 AS samples in total) and examined by high-throughput Illumina-MiSeq sequencing. The results suggested that the microbial community diversity and composition in the influent fluctuated substantially with season, while those in the AS had a relatively more stable pattern throughout the year. The phylogenetic group based assembly mechanisms of AS community were identified by using “Infer Community Assembly Mechanisms by Phylogenetic-bin-based null model (iCAMP)”. The results showed that drift accounted for the largest proportion (52.8%), while homogeneous selection (18.2%) was the most important deterministic process. Deterministic processes dominated in 47 microbial groups (bins), which were also found (~40%) in the AS core taxa dataset. Moreover, the results suggested that Nitrospira were more susceptible to stochastic processes in winter, which may provide a possible explanation for nitrification failure in winter. Network analysis results suggested that the network structure of the AS community could be more stable in summer and autumn. In addition, there were no identical keystone taxa found in different networks (constructed from different plants, sources, and seasons), which supported the context dependency theory. The results of this study deepened our understanding of the microbial ecology in AS systems and provided a foundation for further studies on the community regulation strategy of WWTPs. [Display omitted]