Decreased Expression of Selenoproteins as a Poor Prognosticator of Gastric Cancer in Humans

• Published in: Biological trace element research Vol. 178; no. 1; pp. 22 - 28
• Main Authors: Lan, Xiuwen, Xing, Jun, Gao, Hongyu, Li, Sen, Quan, Lina, Jiang, Yang, Ding, Shaohua, Xue, Yingwei
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.07.2017; Springer Nature B.V
• Subjects: Aged; Biochemistry; Biomarkers; Biomedical and Life Sciences; Biotechnology; Cancer; DNA; Erythrocytes; Female; Females; Gastric cancer; Gene expression; Gene Expression Regulation, Neoplastic; Genes; Humans; Life Sciences; Lymphocytes; Male; Males; Medical prognosis; Middle Aged; Neoplasm Proteins - biosynthesis; Neoplasms; Nucleotide sequence; Nutrition; Oncology; Patients; PCR; Polymerase chain reaction; Prognosis; Protein expression; Selenoproteins; Selenoproteins - biosynthesis; Stomach Neoplasms - blood; Stomach Neoplasms - diagnosis; Tissue; Transcription; Human; Selenium; Selenoproteins; Gastric cancer
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-016-0908-8

The aim of the present study was to analyze the selenoprotein expression levels in gastric cancer patients. We enrolled 40 patients (29 males, 11 females) who were recently diagnosed with gastric cancer and 50 healthy people (30 males, 20 females) as controls. The expression of 25 selenoprotein genes (Dio1, Dio2, Dio3, Gpx1, Gpx2, Gpx3, Gpx4, Gpx6, SelH, SelI, SelK, SelM, SelN, SelO, SelP, SelS, SelT, SelV, SelW, SelX, Sel15, Sps2, TR1, TR2, and TR3) in human gastric cancer tissues, para-carcinoma tissues, adjacent normal gastric tissues, erythrocytes, and lymphocytes in the gastric cancer group and healthy control group was analyzed by qRT-PCR. Here, we showed that among the 25 selenoproteins, 13 selenoproteins in erythrocytes (Gpx1, Gpx4, Sel15, TR1, TR2, SelH, SelK, SelM, SelO, SelS, SelV, SelW, and Sps2), 15 selenoproteins in lymphocytes (Gpx1, Gpx4, Sel15, TR1, TR2, SelH, SelK, SelN, SelO, SelS, SelT, SelV, SelX, SelW, and Sps2) and 13 selenoproteins in gastric cancer and para-carcinoma tissues (Dio1, Dio2, Dio3, Gpx1, Gpx4, Sel15, SelH, SelK, SelM, SelS, SelT, SelW, and Sps2) were significantly decreased ( P  < 0.05) in the gastric cancer group compared to the control group. In summary, the decreasing expression of selenoprotein genes in gastric cancer patients play an important role in the gastric cancer, although further studies are needed to better understand our findings.