A marine inducible prophage vB_CibM-P1 isolated from the aerobic anoxygenic phototrophic bacterium Citromicrobium bathyomarinum JL354

• Published in: Scientific reports Vol. 4; no. 1; p. 7118
• Main Authors: Zheng, Qiang, Zhang, Rui, Xu, Yongle, III, Richard Allen White, Wang, Yu, Luo, Tingwei, Jiao, Nianzhi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19.11.2014; Nature Publishing Group
• Subjects: 45/43; 631/326/1321; 704/172; Aerobiosis - physiology; Alphaproteobacteria - virology; Aquatic Organisms; Bacteria; Bacterial proteins; Base Composition; Deoxyribonucleic acid; DNA; DNA Packaging - physiology; Fibers; Genome Size; Genome, Viral; Genomes; Humanities and Social Sciences; Integration; Lysis; Lysogeny - physiology; Mitomycin - pharmacology; Mitomycin C; Molecular Sequence Annotation; Morphogenesis; multidisciplinary; Myoviridae - classification; Myoviridae - drug effects; Myoviridae - genetics; Myoviridae - ultrastructure; Open Reading Frames; Packaging; Phages; Phototrophic bacteria; Phototrophic Processes - physiology; Phylogeny; Prophages - classification; Prophages - drug effects; Prophages - genetics; Prophages - ultrastructure; Science; Tail fiber protein; Viral Proteins - genetics; Virion - physiology; Virus Activation - drug effects; Virus Integration - physiology; Virus-like particles
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep07118

A prophage vB_CibM-P1 was induced by mitomycin C from the epipelagic strain Citromicrobium bathyomarinum JL354, a member of the alpha-IV subcluster of marine aerobic anoxygenic phototrophic bacteria (AAPB). The induced bacteriophage vB_CibM-P1 had Myoviridae -like morphology and polyhedral heads (approximately capsid 60–100 nm) with tail fibers. The vB_CibM-P1 genome is ~38 kb in size, with 66.0% GC content. The genome contains 58 proposed open reading frames that are involved in integration, DNA packaging, morphogenesis and bacterial lysis. VB_CibM-P1 is a temperate phage that can be directly induced in hosts. In response to mitomycin C induction, virus-like particles can increase to 7 × 10 9 per ml, while host cells decrease an order of magnitude. The vB_CibM-P1 bacteriophage is the first inducible prophage from AAPB.