Regenerative Potential of Stem and Progenitor Cells from Ischemic Testes of C57Bl/6 Mice in Culture and in the Model of Spermatogenesis Suppression Caused by Busulfan

• Published in: Bulletin of experimental biology and medicine Vol. 162; no. 3; pp. 400 - 405
• Main Authors: Skurikhin, E. G., Pakhomova, A. V., Pershina, O. V., Ermolaeva, L. A., Ermakova, N. N., Krupin, V. A., Pan, E. S., Kudryashova, A. I., Rybalkina, O. Yu, Zhdanov, V. V., Goldberg, V. E., Dygai, A. M.
• Format: Journal Article
• Language: English
• Published: New York Springer US 2017; Springer Nature B.V
• Subjects: Animals; Antigens, CD - genetics; Biomedical and Life Sciences; Biomedicine; Busulfan; Busulfan - toxicity; CD45 antigen; CD90 antigen; Cell Biology; Cell culture; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cell self-renewal; Endothelial Cells - drug effects; Endothelial Cells - metabolism; Endothelial Cells - pathology; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Epithelial Cells - pathology; Epithelium; Gene Expression; Internal Medicine; Ischemia; Ischemia - metabolism; Ischemia - pathology; Laboratory Medicine; Leydig cells; Leydig Cells - drug effects; Leydig Cells - metabolism; Leydig Cells - pathology; Ligation; Male; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - metabolism; Mesenchymal Stromal Cells - pathology; Mice; Mice, Inbred C57BL; Morphology and Pathomorphology; Pathology; Regeneration - drug effects; Rodents; Seminiferous Tubules - drug effects; Seminiferous Tubules - metabolism; Seminiferous Tubules - pathology; Spermatic Cord - blood supply; Spermatic Cord - surgery; Spermatogenesis; Spermatogenesis - drug effects; Spermatogonia - drug effects; Spermatogonia - metabolism; Spermatogonia - pathology; Stem Cell Transplantation; Stem cells; Testes; Testosterone; Testosterone - biosynthesis; Tubules; busulfan; epithelial precursors; spermatogonial stem cells; endothelial precursors; Sca-1 + cells
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-017-3625-1

The regenerative potential of stem and progenitor cells from ischemic testes of C57Bl/6 mice was studied in vitro (cell culture) and in vivo (mouse model of busulfan-induced suppression of spermatogenesis). Spermatogonial stem cells with phenotypes CD117 — CD90 + and CD51 — CD24 + CD52 + from ischemic testes demonstrated 33-fold and 7-fold increments of cell mass and generated colonies in vitro . Epithelial (CD45 — CD31 — Sca-1 + CD49f + ) and endothelial (CD45 — CD31 + ) precursors exhibited lower self-renewal capacity. On day 30 after injection of stem and progenitor cells from ischemic testes to the rete testis zone of the testes of busulfantreated animals, an increase in the count of CD117 — CD90 + spermatogonial stem cells, total count, and mobile sperm count in the testes of recipient mice was observed. In addition, we observed an increase in Sca-1 + cell count, recovery of the spermatogenic epithelium in the seminiferous tubules, and appearance of immature Leydig cells in “busulfan” testes; the level of tissue testosterone and fertility index also increased.