Partially reduced graphene oxide based FRET on fiber-optic interferometer for biochemical detection

Fluorescent resonance energy transfer (FRET) with naturally exceptional selectivity is a powerful technique and widely used in chemical and biomedical analysis. However, it is still challenging for conventional FRET to perform as a high sensitivity compact sensor. Here we propose a novel ‘ FRET on F...

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Published inScientific reports Vol. 6; no. 1; p. 23706
Main Authors Yao, B. C., Wu, Y., Yu, C. B., He, J. R., Rao, Y. J., Gong, Y., Fu, F., Chen, Y. F., Li, Y. R.
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 24.03.2016
Nature Publishing Group
Subjects
132/124
140/133
639/624/1075/1083
639/925/918/1054
Biosensing Techniques
Detection limits
Dopamine
Energy transfer
Fiber Optic Technology
Fluorescence resonance energy transfer
Fluorescence Resonance Energy Transfer - methods
Graphite - chemistry
Humanities and Social Sciences
Limit of Detection
multidisciplinary
Optics
Oxides - chemistry
Phase shift
Science
Single-stranded DNA
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Summary:Fluorescent resonance energy transfer (FRET) with naturally exceptional selectivity is a powerful technique and widely used in chemical and biomedical analysis. However, it is still challenging for conventional FRET to perform as a high sensitivity compact sensor. Here we propose a novel ‘ FRET on Fiber ’ concept, in which a partially reduced graphene oxide (prGO) film is deposited on a fiber-optic modal interferometer, acting as both the fluorescent quencher for the FRET and the sensitive cladding for optical phase measurement due to refractive index changes in biochemical detection. The target analytes induced fluorescence recovery with good selectivity and optical phase shift with high sensitivity are measured simultaneously. The functionalized prGO film coated on the fiber-optic interferometer shows high sensitivities for the detections of metal ion, dopamine and single-stranded DNA (ssDNA), with detection limits of 1.2 nM, 1.3 μM and 1 pM, respectively. Such a prGO based ‘FRET on fiber’ configuration, bridging the FRET and the fiber-optic sensing technology, may serve as a platform for the realization of series of integrated ‘ FRET on Fiber ’ sensors for on-line environmental, chemical, and biomedical detection, with excellent compactness, high sensitivity, good selectivity and fast response
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ISSN:2045-2322
2045-2322
DOI:10.1038/srep23706