Expression Strategy of the Potato Virus X Triple Gene Block

• Published in: Journal of general virology Vol. 72; no. 8; pp. 2039 - 2042
• Main Authors: Morozov, S. Yu, Miroshnichenko, N. A, Solovyev, A. G, Fedorkin, O. N, Zelenina, D. A, Lukasheva, L. I, Karasev, A. V, Dolja, V. V, Atabekov, J. G
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.08.1991; Society for General Microbiology
• Subjects: Base Sequence; Biological and medical sciences; DNA, Viral; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Viral; Genetics; Microbiology; Molecular Sequence Data; Plant Viruses - genetics; potato virus X; Restriction Mapping; RNA Viruses - genetics; RNA, Viral - genetics; Solanum tuberosum - microbiology; Virology; Virus; In vitro translation; Gene; Plant pathogen; Potato virus X; Potexvirus; Overlapping sequence; Gene expression
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-72-8-2039

1 A. N. Belozersky Laboratory of Molecular Biology and Department of Virology, Moscow State University, Moscow 119899 and 2 Institute of Microbiology, Academy of Sciences of the USSR, Moscow 117811, U.S.S.R. The mode of expression of the overlapping genes of the triple block positioned internally in potato virus X (PVX) RNA was examined. The results of In vitro translation of synthetic RNA transcripts and natural PVX-specific methylmercuric hydroxide-denatured dsRNAs suggest that the 25K protein is expressed as a single translation product of the 2·1 kb subgenomic (sg) RNA and that both the 12K and 8K proteins are expressed from the same 1·4 kb sgRNA. Received 4 January 1991; accepted 17 April 1991.