Energy metabolism regulated by HDAC inhibitor attenuates cardiac injury in hemorrhagic rat model

• Published in: Scientific reports Vol. 6; no. 1; p. 38219
• Main Authors: Kuai, Qiyuan, Wang, Chunyan, Wang, Yanbing, Li, Weijing, Zhang, Gongqing, Qiao, Zhixin, He, Min, Wang, Xuanlin, Wang, Yu, Jiang, Xingwei, Su, Lihua, He, Yuezhong, Ren, Suping, Yu, Qun
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 02.12.2016; Nature Publishing Group
• Subjects: 13/109; 13/44; 13/89; 14/28; 14/34; 38/77; 42; 59; 631/45/612/1240; 692/4019; 692/699/578; 82; Alternative energy sources; Animals; ATP; Cell Line; Contraction; Disease Models, Animal; Electron transport; Energy metabolism; Energy Metabolism - drug effects; Gene expression; Heart diseases; Hemorrhage; Hemorrhage - drug therapy; Hemorrhage - metabolism; Hemorrhage - pathology; Histone deacetylase; Histone Deacetylase Inhibitors - pharmacology; Humanities and Social Sciences; Hypoxia; L-Lactate dehydrogenase; Lactic acid; Leukemia; Male; Mcl-1 protein; Metabolism; Mitochondria; multidisciplinary; Myocardial Ischemia - metabolism; Myocardial Ischemia - pathology; Myocardial Ischemia - prevention & control; Myocardium - metabolism; Myocardium - pathology; Rats; Rats, Wistar; Rodents; Science; Ultrastructure; Valproic acid; Valproic Acid - pharmacology
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep38219

A disturbance of energy metabolism reduces cardiac function in acute severe hemorrhagic patients. Alternatively, adequate energy supply reduces heart failure and increases survival. However, the approach to regulating energy metabolism conductive to vital organs is limited, and the underlying molecular mechanism remains unknown. This study assesses the ability of histone deacetylase inhibitors (HDACIs) to preserve cardiac energy metabolism during lethal hemorrhagic injury. In the lethally hemorrhagic rat and hypoxic myocardial cells, energy metabolism and heart function were well maintained following HDACI treatment, as evident by continuous ATP production with normal cardiac contraction. Valproic acid (VPA) regulated the energy metabolism of hemorrhagic heart by reducing lactate synthesis and protecting the mitochondrial ultrastructure and respiration, which were attributable to the inhibition of lactate dehydrogenase A activity and the increased myeloid cell leukemia-1 (mcl-1) gene expression, ultimately facilitating ATP production and consumption. MCL-1, the key target of VPA, mediated this cardioprotective effect under acute severe hemorrhage conditions. Our results suggest that HDACIs promote cardioprotection by improving energy metabolism during hemorrhagic injury and could therefore be an effective strategy to counteract this process in the clinical setting.