Fabrication of two distinct hydroxyapatite coatings and their effects on MC3T3-E1 cell behavior

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 171; pp. 40 - 48
• Main Authors: Pang, Shumin, He, Yuan, He, Ping, Luo, Xueshi, Guo, Zhenzhao, Li, Hong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2018
• Subjects: Adsorption; alkaline phosphatase; Animals; bioactive properties; biocompatible materials; bone formation; Cell Differentiation - drug effects; Cell Line; cell proliferation; Cell Proliferation - drug effects; Cell Survival - drug effects; coatings; collagen; colloids; Durapatite - chemistry; Durapatite - pharmacology; electrolytes; fibronectins; Flake-like; gene expression; hydrophilicity; Hydrothermal-electrodeposition; Hydroxyapatite; MC3T3-E1 cells; Mice; osteocalcin; Particle Size; reverse transcriptase polymerase chain reaction; Rod-like; roughness; Surface Properties; Titanium - chemistry; Titanium - pharmacology; transmission electron microscopy; X-ray diffraction; Rod-like; Flake-like; Hydroxyapatite; Hydrothermal-electrodeposition; MC3T3-E1 cells
• ISSN: 0927-7765; 1873-4367; 1873-4367
• DOI: 10.1016/j.colsurfb.2018.06.046

Two distinct hydroxyapatite (HAP) with ordered rod-like and flower plate-like HAP crystals were deposited directly on titanium surface and MC3T3-E1 cells responds on them were investigated. [Display omitted] •Rod-like and flake-like hydroxyapatite coatings were fabricated.•With different crystal orientation, surface roughness and wettability.•Rod-like one prefers cell spreading, proliferation and osteogenic differentiation.•Might cause topography-dependent coordination with biomolecules. How the surface topography of hydroxyapatite (HAP) coatings remodels hard tissue is of utmost importance and a contributing factor to ambiguity regarding this subject. Here, HAP coatings with different topographies of a rod-like nanoarray with c-axis orientation and a flake-like micro-flower array with a(b)-axis orientation on a Ti substrate were synthesized via hydrothermal-electrodeposition by controlling the concentration of electrolytes. XRD, TEM and SAED analyses indicated that the rod-like HAP nanoarray was predominant with an orientation of (001), while the HAP micro-flower samples were based on an orientation of (100). Compared to the flake-like HAP, the rod-like nanoarray HAP possessed better hydrophilic properties and lower roughness, which not only enhanced adsorption of specific fibronectin proteins but also promoted the spreading and growth of MC3T3-E1 cells. Runx2, alkaline phosphatase, collagen and osteocalcin were also analyzed by RT-PCR on the two distinctive HAP-coated samples. MC3T3-E1 cells on the rod-like nanoarray coating had higher osteo-related gene expression. This finding suggested that the ordered assembly structure of the HAP might cause topography-dependent coordination with biomolecules for enhancing osteoblast-like cell proliferation and osteogenic differentiation. This study provided an understanding of the surface’s features for biomaterials to ensure better bioactivity.