Isolation and preliminary characterization of a 2-chlorobenzoate degrading Pseudomonas

Pseudomonas sp. strain B-300, which is able to utilize 2-chlorobenzoic acid, was isolated from a soil sample by enrichment culture. This strain was shown to grow on 2-chlorobenzoic acid and to completely degrade the substrate with concomitant chlorine ion release. Concentrations of 2-chlorobenzoic a...

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Bibliographic Details
Published inCanadian journal of microbiology Vol. 35; no. 4; p. 439
Main Authors Sylvestre, M, Mailhiot, K, Ahmad, D, Massé, R
Format Journal Article
LanguageEnglish
Published Canada 01.04.1989
Subjects
Biodegradation, Environmental
Chemical Phenomena
Chemistry
Chlorobenzoates - metabolism
Gas Chromatography-Mass Spectrometry
Pseudomonas - growth & development
Pseudomonas - isolation & purification
Pseudomonas - metabolism
Soil Microbiology
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Summary:Pseudomonas sp. strain B-300, which is able to utilize 2-chlorobenzoic acid, was isolated from a soil sample by enrichment culture. This strain was shown to grow on 2-chlorobenzoic acid and to completely degrade the substrate with concomitant chlorine ion release. Concentrations of 2-chlorobenzoic acid higher than 0.5% (w/v) were toxic to the cells. Our study also suggested that in the presence of glucose, 2-chlorobenzoic acid is converted to catechol or chlorocatechol; these are in turn transformed to muconic and chloromuconic acid, respectively, suggesting a repression by glucose of some of the degradation pathway enzymes. A similar scheme was already described for 3-chlorobenzoate degradation by pAC25 plasmid.
ISSN:0008-4166
1480-3275
DOI:10.1139/m89-067