Preparation, Characterization, and Antioxidant Activities of Extracts from Amygdalus persica L. Flowers

• Published in: Molecules (Basel, Switzerland) Vol. 29; no. 3; p. 633
• Main Authors: Yu, Qingtao, Li, Wenzhi, Liang, Ming, Li, Guohu, Wu, Zhuoyan, Long, Jieyi, Yuan, Chanling, Mei, Wenjie, Xia, Xiaole
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.01.2024
• Subjects: Amygdalus persica L. flowers; Animals; antioxidant activity; Antioxidants; Antioxidants - chemistry; Flavonoids; Flowers - chemistry; Food; Free radicals; Lipopolysaccharides; Melanins - analysis; Molecular weight; Monophenol Monooxygenase; Oxidation; Oxidative stress; polysaccharide; Proteins; Signal transduction; Spectrum analysis; structural characterization; Toxicity; Water - analysis; Zebrafish; Amygdalus persica L. flowers; polysaccharide; structural characterization; antioxidant activity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29030633

A novel water-soluble L. flowers polysaccharide (APL) was successfully isolated and purified from L. flowers by hot water extraction. Its chemical components and structure were analyzed by IR, GC-MS, and HPLC. APL consisted of rhamnose, arabinose, mannose and glucose in a molar ratio of 0.17:0.034:1.0:0.17 with an average molecular weight of approximately 208.53 kDa and 15.19 kDa. The antioxidant activity of APL was evaluated through radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH), 3-ethylbenzthiazoline-6-sulfonic acid (ABTS), Hydroxyl radical scavenging, Superoxide radical scavenging, and the reducing power activity was also determined in vitro. Besides, in vivo antioxidant experiment, zebrafish ( ) embryos were treated with different concentrations of APL and then exposed to LPS to induce oxidative stress. Treatment with APL at 50 or 100 µg/mL significantly reduced LPS-induced oxidative stress in the zebrafish, demonstrating the strong antioxidant activity of APL. Moreover, the effect of APL on zebrafish depigmentation was tested by analyzing the tyrosinase activity and melanin content of zebrafish embryos. APL showed a potential reduction in the total melanin content and tyrosinase activity after treatment. This work provided important information for developing a potential natural antioxidant in the field of cosmetics and food.