Hematological, biochemical and ionoregulatory responses of Indian major carp Catla catla during chronic sublethal exposure to inorganic arsenic

• Published in: Chemosphere (Oxford) Vol. 82; no. 7; pp. 977 - 985
• Main Authors: Lavanya, Subramani, Ramesh, Mathan, Kavitha, Chokkalingam, Malarvizhi, Annamalai
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.02.2011; Elsevier
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Animals; Applied ecology; Arsenic trioxide; Arsenicals; Biochemical Phenomena; Biological and medical sciences; Biomarkers - metabolism; Blood Chemical Analysis; Blood Glucose - metabolism; Catla catla; Cyprinidae - metabolism; Cyprinidae - physiology; Ecotoxicology, biological effects of pollution; Erythrocyte Count; Erythrocyte Indices - drug effects; Fresh water ecosystems; Fundamental and applied biological sciences. Psychology; General aspects; Gills - drug effects; Gills - metabolism; Hematocrit - methods; Hematologic Tests; Hematology; Hemoglobins - metabolism; India; L-Lactate Dehydrogenase - metabolism; Lethal Dose 50; Leukocyte Count; Na +/K +-ATPase; Oxides - toxicity; Sodium-Potassium-Exchanging ATPase - metabolism; Synecology; Toxicity Tests, Acute; Transaminases; Water Pollutants, Chemical - toxicity; India; Arsenic trioxide; Na +/K +-ATPase; Hematology; Transaminases; Catla catla; Arsenic; Enzyme; Ionic regulation; Transferases; ATPase; K; Heavy metal; Freshwater environment; Trace element; Aquatic environment; Carcinogen; Pollutant; Vertebrata; Chronic; Na; Pisces; Hydrolases; exchanging ATPase; Poison
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2010.10.071

In the present study the acute toxicity of arsenic trioxide in fingerlings of Catla catla an Indian major carp was evaluated with renewal bioassay method. The median lethal concentration of arsenic trioxide to the fish C. catla for 96 h was found to be 20.41 ppm (with 95% confidence limits). From this a non-lethal dose of (2.041 ppm; 1/10th of LC 50 96 value) was selected and fingerlings were exposed to 35 d and hematological, biochemical and ionoregulatory responses were studied at days 7, 14, 21, 28 and 35. Arsenic trioxide produced a significant increase in hemoglobin, hematocrit, WBC count, plasma GPT levels and reduction in RBC count, plasma sodium, chloride, potassium, glucose, protein, GOT, LDH levels as compared to the control group. Gill Na +/K +-ATPase activity was influenced by arsenic trioxide exposure. A biphasic response was noted in the value of MCH and MCV. However the MCHC level was not altered in arsenic trioxide treated fish throughout the study period. Results of the present investigation suggest that arsenic trioxide affects the hematological, biochemical and ionoregulatory parameters of fish and alterations of these parameters can be useful in environmental biomonitoring of arsenic contamination.