Differentially expressed circulating LncRNAs and mRNA identified by microarray analysis in obese patients

• Published in: Scientific reports Vol. 6; no. 1; p. 35421
• Main Authors: Sun, Jia, Ruan, Yuting, Wang, Ming, Chen, Rongping, Yu, Na, Sun, Lei, Liu, Tiemin, Chen, Hong
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.10.2016; Nature Publishing Group
• Subjects: 38/61; 631/1647/2017/2079; 692/163/2743/393; Adipose Tissue - metabolism; Adult; Biomarkers; Body mass; Body mass index; Body weight loss; Cross-Sectional Studies; Fasting; Female; Gene Expression Profiling; Gene Expression Regulation; Gene Regulatory Networks; Genomes; GTP-binding protein; Hip; Homeostasis; Human subjects; Humanities and Social Sciences; Humans; Insulin; Laboratory testing; Longitudinal Studies; Male; mRNA; multidisciplinary; Obesity; Obesity - blood; Obesity - genetics; Oligonucleotide Array Sequence Analysis; Polymerase chain reaction; RNA, Long Noncoding - blood; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Science; Weight control; Weight Loss - genetics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep35421

Circulating long non-coding RNAs (lncRNAs) serve as valuable biomarkers in a number of human diseases. However, lncRNA biomarkers have yet to be identified in obesity. We aim to characterize circulating lncRNA expression in obese and non-obese human subjects. First, we assessed the genome-wide circulating lncRNA expression profiles in blood from 3 obese and 3 non-obese human subjects. We found a significant decrease in circulating levels of three lncRNAs (lncRNA-p5549, lncRNA-p21015 and lncRNA-p19461) in obese human subjects only. Next, using RT-PCR we measured the expression levels of these three lncRNAs in 33 obese and 33 non-obese human subjects and found similar differences. Moreover, we found a negative correlation between circulating levels of these three lncRNAs and body mass index (BMI), waist circumference, waist to hip ratio and fasting insulin. There was also a significant negative correlation between expression of lncRNA-p19461 and homeostasis model assessment-estimated insulin resistance. Finally, we tested the circulating levels of these three lncRNAs in 8 obese human subjects after a 12-week diet-induced weight loss program. We found that only lncRNA-p19461 expression level significantly increased. In summary, circulating lncRNAs are deregulated in obesity. Weight loss–induced changes in this profile support this observation and suggest a potential mechanistic relevance.