Chondroitin sulfate and glucosamine sulfate associated to photobiomodulation prevents degenerative morphological changes in an experimental model of osteoarthritis in rats

• Published in: Lasers in medical science Vol. 33; no. 3; pp. 549 - 557
• Main Authors: Sanches, Marcella, Assis, Lívia, Criniti, Cyntia, Fernandes, Danilo, Tim, Carla, Renno, Ana Claudia Muniz
• Format: Journal Article
• Language: English
• Published: London Springer London 01.04.2018; Springer Nature B.V
• Subjects: Animals; Arthritis; Biocompatibility; Cartilage; Cartilage (articular); Cartilage diseases; Chondrocytes; Chondrocytes - drug effects; Chondrocytes - pathology; Chondrocytes - radiation effects; Chondroitin sulfate; Chondroitin Sulfates - pharmacology; Chondroitin Sulfates - therapeutic use; Collagen (type II); Collagen Type II - metabolism; Combined Modality Therapy; Combined treatment; Dentistry; Disease Models, Animal; Glucosamine; Glucosamine - pharmacology; Glucosamine - therapeutic use; Immunohistochemistry; Inflammation; Interleukin 10; Interleukin-10 - metabolism; Interleukin-1beta - metabolism; Knee; Lasers; Light therapy; Low-Level Light Therapy; Male; Medicine; Medicine & Public Health; Morphology; Optical Devices; Optics; Original Article; Osteoarthritis; Osteoarthritis - drug therapy; Osteoarthritis - metabolism; Osteoarthritis - pathology; Osteoarthritis - radiotherapy; Photonics; Physical characteristics; Quality; Quantum Optics; Rats; Rats, Wistar; Rodents; Sulfates; Surgery; Glucosamine sulfate; Chondroitin sulfate; Knee osteoarthritis; Articular cartilage; Photobiomodulation
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-017-2401-9

The aim of this study was to compare the effects of combined treatment with chondroitin sulfate and glucosamine sulfate (CS/Gl) and photobiomodulation (PBM) on the degenerative process related to osteoarthritis (OA) in the articular cartilage in rats. Forty male Wistar rats were randomly divided into four groups: OA control group (CG); OA animals submitted to PBM treatment (PBM); OA animals submitted to CS/Gl treatment (CS/Gl); OA submitted to CS/GS associated with PBM treatments (GS/Gl + PBM). The CS/Gl started 48 h after the surgery, and they were performed for 29 consecutive days. Moreover, PBM was performed after the CS/Gl administration on the left joint. Morphological characteristics and immunoexpression of interleukin 10 (IL-10) and 1 beta (IL-1β) and collagen type II (Col II) of the articular cartilage were evaluated. The results showed that all treated groups (CS/Gl and PBM) presented attenuation signs of degenerative process (measured by histopathological analysis) and lower density chondrocytes [PBM ( p  = 0.0017); CS/Gl ( p  = 0.0153) and CS/Gl + PBM ( p  = 0.002)]. Additionally, CS/Gl [associated ( p  = 0.0089) or not with PBM ( p  = 0.0059)] showed significative lower values for OARSI grade evaluation. Furthermore, CS/GS + PBM decreased IL-1β protein expression ( p  = 0.0359) and increased IL-10 ( p  = 0.028) and Col II imunoexpression ( p  = 0.0204) compared to CG. This study showed that CS/Gl associated with PBM was effective in modulating inflammatory process and preventing the articular tissue degradation in the knees OA rats.