Cinnamon polyphenols regulate S100β, sirtuins, and neuroactive proteins in rat C6 glioma cells

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Vol. 30; no. 2; pp. 210 - 217
• Main Authors: Qin, Bolin, Ph.D., M.D, Panickar, Kiran S., Ph.D, Anderson, Richard A., Ph.D
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.02.2014; Elsevier
• Subjects: Animals; bcl-X Protein - genetics; bcl-X Protein - metabolism; Biological and medical sciences; Cell Line, Tumor; Cinnamomum zeylanicum - chemistry; Cytokines - genetics; Cytokines - metabolism; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Gastroenterology and Hepatology; Glioma - metabolism; Mitogen-Activated Protein Kinases - genetics; Mitogen-Activated Protein Kinases - metabolism; Phospho-NF-κB and Bcl-xl; Phosphorylation; Polyphenols; Polyphenols - pharmacology; Rats; S100 Calcium Binding Protein beta Subunit - genetics; S100 Calcium Binding Protein beta Subunit - secretion; S100β; Sirtuin 1 - genetics; Sirtuin 1 - metabolism; Sirtuin 2 - genetics; Sirtuin 2 - metabolism; Sirtuins; Spice; Transcription Factor RelA - genetics; Transcription Factor RelA - metabolism; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - metabolism; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism; Up-Regulation; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Sirtuins; Polyphenols; Spice; S100β; Phospho-NF-κB and Bcl-xl; Glial cell; Nutrition; Rat; Neuroglia; Rodentia; In vitro; Protein; Micronutrient; Vertebrata; Polyphenol; Regulation(control); Mammalia; Cell line; Animal; Proanthocyanidin
• ISSN: 0899-9007; 1873-1244
• DOI: 10.1016/j.nut.2013.07.001

Abstract Objective Increasing evidence suggests that cinnamon has many health benefits when used in herbal medicine and as a dietary ingredient. The aim of this study was to investigate the effects of an aqueous extract of cinnamon, high in type A polyphenols, on molecular targets in rat C6 glioma cells that underlie their protective effects. Methods C6 rat glioma cells were seeded in 35-mm culture dishes or six-well plates, then were incubated with cinnamon polyphenols at doses of 10 and 20 μg/mL for 24 h. The targeting protein expression, secretion, and phosphorylation were evaluated by immunoprecitation/immunoblotting and immunofluorescence imaging. Results Cinnamon polyphenols significantly enhanced secretion of S100β, a Ca2+ -binding protein, and increased intracellular S100β expression after 24 h of incubation, in rat C6 glioma cells. Cinnamon polyphenols also enhanced protein levels of sirtuin 1, 2, and 3, deacetylases important in cell survival, and the tumor suppressor protein, p53, and inhibited the inflammatory factors, tumor necrosis factor alpha, and phospho-p65, a subunit of nuclear factor-κβ. Cinnamon polyphenols also up-regulated levels of phospho-p38, extracellular signal-regulated protein and mitogen-activated protein and kinase-activated protein kinases that may be important for prosurvival functions. Conclusion Our results indicate that the effects of cinnamon polyphenols on upregulating prosurvival proteins, activating mitogen-activated protein kinase pathways, and decreasing proinflammatory cytokines may contribute to their neuroprotective effects.