Discovery and verification of panels of T-lymphocyte proteins as biomarkers of Parkinson's disease

The diagnosis of Parkinson's disease (PD) is currently based on the clinical evaluation of extrapyramidal signs with a considerable error rate. The identification of specific markers might allow PD diagnosis before the onset of classical motor symptoms. By two-dimensional electrophoresis we ide...

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Bibliographic Details
Published inScientific reports Vol. 2; no. 1; p. 953
Main Authors Alberio, Tiziana, Pippione, Agnese C., Zibetti, Maurizio, Olgiati, Simone, Cecconi, Daniela, Comi, Cristoforo, Lopiano, Leonardo, Fasano, Mauro
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 11.12.2012
Nature Publishing Group
Subjects
631/378/340
631/61/475
692/53/2421
692/699/375/365/1718
Age
Biomarkers - analysis
Blotting, Western
Electrophoresis, Gel, Two-Dimensional
Extrapyramidal system
Fibrinogen
Fibrinogen - analysis
Humanities and Social Sciences
Humans
Isoforms
Lymphocytes
Lymphocytes T
Motor task performance
Movement disorders
multidisciplinary
Neurodegenerative diseases
Parkinson Disease - diagnosis
Parkinson's disease
Prediction models
Protein Isoforms - analysis
Proteins
Proteome - analysis
Proteomes
Science
T-Lymphocytes - chemistry
Transaldolase
Transaldolase - analysis
Western blotting
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Summary:The diagnosis of Parkinson's disease (PD) is currently based on the clinical evaluation of extrapyramidal signs with a considerable error rate. The identification of specific markers might allow PD diagnosis before the onset of classical motor symptoms. By two-dimensional electrophoresis we identified proteome alterations in T-lymphocytes of 17 control subjects and 15 PD patients. The observed changes were used to build predictive models that were verified by the leave-one-out cross-validation. We further built two functions able to stage the subjects. We chose to verify by Western blotting the identity of spots corresponding to β-fibrinogen and transaldolase, two recurrent proteins in six out of 20 spots. β-Fibrinogen levels are lowered in PD patients, whereas a heavy transaldolase set of isoforms was more abundant. Eventually, we identified a list of seven proteins showing different levels in early-onset with respect to late-onset PD patients.
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ISSN:2045-2322
2045-2322
DOI:10.1038/srep00953