Amperometric enzyme sensor for the rapid determination of histamine

• Published in: Analytical methods Vol. 11; no. 9; pp. 1264 - 1269
• Main Authors: Torre, Ricarda, Costa-Rama, Estefanía, Lopes, Paula, Nouws, Henri P. A, Delerue-Matos, Cristina
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 2019
• Subjects: Amperometry; Bovine serum albumin; Crosslinking; Electrical measurement; Electrodes; Enzymes; Feasibility studies; Fish; Glutaraldehyde; Histamine; Portable document format; Reproducibility; Response time; Sensors; Serum albumin; Spoilage
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/c8ay02610f

The concentration of histamine, a biogenic amine, in fish is considered a fish spoilage indicator. Therefore, the development of a rapid-response and portable tool that allows its on-site analysis is very interesting since the consumption of high amounts of histamine causes scombroid poisoning. Thus, in this work a simple enzymatic sensor for the determination of histamine, based on a screen-printed carbon electrode and the enzyme diamine oxidase, was developed. The enzyme was immobilized on the electrode surface through a simple cross-linking procedure employing glutaraldehyde and bovine serum albumin. Chronoamperometry was used as the detection technique; the sensor showed a short response time (60 s, −0.3 V) and the measurements were performed by only using 40 μL of sample solution. The sensor provides a useful linear range (between 1 and 75 mg L −1 in fish extract), excellent reproducibility (RSD = 2.6%) and is reusable for up to 7 measurements. The feasibility of the sensor was tested performing histamine analysis in fish extracts achieving recovery values of 103%. A simple sensor combining screen-printed carbon electrodes and diamine oxidase is developed for histamine detection.