Assessment of a Standardized ROS Production Profile in Humans by Electron Paramagnetic Resonance

• Published in: Oxidative medicine and cellular longevity Vol. 2012; no. 2012; pp. 1 - 10
• Main Authors: Mrakic-Sposta, Simona, Gussoni, Maristella, Montorsi, Michela, Porcelli, Simone, Vezzoli, Alessandra
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Puplishing Corporation 01.01.2012; Hindawi Publishing Corporation
• Subjects: Antioxidants - administration & dosage; Antioxidants - pharmacology; Biomarkers - metabolism; Electron Spin Resonance Spectroscopy - methods; Electron Spin Resonance Spectroscopy - standards; Exercise; Female; Humans; Male; Middle Aged; Oxidative Stress - drug effects; Protein Carbonylation - drug effects; Reactive Oxygen Species - metabolism; Reference Standards; Reproducibility of Results; Thiobarbituric Acid Reactive Substances - metabolism; Thioctic Acid - administration & dosage; Thioctic Acid - pharmacology; Time Factors; Young Adult

Despite the growing interest in the role of reactive oxygen species (ROS) in health and disease, reliable quantitative noninvasive methods for the assessment of oxidative stress in humans are still lacking. EPR technique, coupled to a specific spin probe (CMH: 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tet...