Sustainable, Rapid Synthesis of Bright-Luminescent CuInS2-ZnS Alloyed Nanocrystals: Multistage Nano-xenotoxicity Assessment and Intravital Fluorescence Bioimaging in Zebrafish-Embryos

Near-infrared (NIR) luminescent CuInS 2 -ZnS alloyed nanocrystals (CIZS-NCs) for highly fluorescence bioimaging have received considerable interest in recent years. Owing, they became a desirable alternative to heavy-metal based-NCs and organic dyes with unique optical properties and low-toxicity fo...

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Published inScientific reports Vol. 6; no. 1; p. 26078
Main Authors Chetty, S. Shashank, Praneetha, S., Basu, Sandeep, Sachidanandan, Chetana, Murugan, A. Vadivel
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 18.05.2016
Nature Publishing Group
Subjects
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Cell lines
Crystals
Embryos
Fluorescence
Humanities and Social Sciences
I.R. radiation
multidisciplinary
Nanocrystals
Optical properties
Science
Spatial discrimination
Teratogenicity
Toxicity
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Summary:Near-infrared (NIR) luminescent CuInS 2 -ZnS alloyed nanocrystals (CIZS-NCs) for highly fluorescence bioimaging have received considerable interest in recent years. Owing, they became a desirable alternative to heavy-metal based-NCs and organic dyes with unique optical properties and low-toxicity for bioimaging and optoelectronic applications. In the present study, bright and robust CIZS-NCs have been synthesized within 5 min, as-high-as 230 °C without requiring any inert-gas atmosphere via microwave-solvothermal (MW-ST) method. Subsequently, the in vitro and in vivo nano-xenotoxicity and cellular uptake of the MUA-functionalized CIZS-NCs were investigated in L929, Vero, MCF7 cell lines and zebrafish-embryos. We observed minimal toxicity and acute teratogenic consequences upto 62.5 μg/ml of the CIZS-NCs in zebrafish-embryos. We also observed spontaneous uptake of the MUA-functionalized CIZS-NCs by 3 dpf older zebrafish-embryos that are evident through bright red fluorescence-emission at a low concentration of 7.8 μg/mL. Hence, we propose that the rapid, low-cost, large-scale “sustainable” MW-ST synthesis of CIZS-NCs, is an ideal bio-nanoprobe with good temporal and spatial resolution for rapid labeling, long-term in vivo tracking and intravital-fluorescence-bioimaging (IVBI).
ISSN:2045-2322
2045-2322
DOI:10.1038/srep26078