Overexpressing a putative aquaporin gene from wheat [Triticum aestivum], TaNIP, enhances salt tolerance in transgenic Arabidopsis
High soil salinity is a major abiotic stress in plant agriculture worldwide. Here, we report the characterization of a novel aquaporin gene TaNIP (Triticum asetivum L. nodulin 26-like intrinsic protein), which was involved in salt tolerance pathways in plants. TaNIP was identified and cloned through...
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Published in | Plant and cell physiology Vol. 51; no. 5; pp. 767 - 775 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Japan
Oxford University Press
01.05.2010
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Subjects | |
Online Access | Get full text |
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Summary: | High soil salinity is a major abiotic stress in plant agriculture worldwide. Here, we report the characterization of a novel aquaporin gene TaNIP (Triticum asetivum L. nodulin 26-like intrinsic protein), which was involved in salt tolerance pathways in plants. TaNIP was identified and cloned through the gene chip expression analysis of a salt-tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription-PCR (Q-RT-PCR) was used to detect TaNlP expression under salt, drought, cold and ABA treatment. The overexpression of TaNIP in transgenic Arabidopsis produced higher salt tolerance than wild-type plants. Localization analysis showed that TaNIP proteins tagged with green fluorescent protein (GFP) were localized to the cell plasma membrane. Under salt stress treatment, TaNIP-overexpressing Arabidopsis accumulated higher Ksup(+), Casup(2+) and proline contents and lower Nasup(+) level than the wild-type plants. The overexpression of TaNIP in transgenic Arabidopsis also up-regulated the expression of a number of stress-associated genes. Our results suggest that TaNIP plays an important role in salt tolerance in Arabidopsis and can also enhance plants' tolerance to other abiotic stresses. |
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Bibliography: | 2010003543 F60 ArticleID:pcq036 istex:56EFCAA82D7ED0FF4148A3CCD65967A3C9D36041 ark:/67375/HXZ-F6QQLB2Q-Z ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0032-0781 1471-9053 |
DOI: | 10.1093/pcp/pcq036 |